Generation and characterization of gemcitabine-resistant pancreatic carcinoma-derived cell lines

• Grant number: 19/04420-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2019
• End date: December 31, 2019
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Eduardo Moraes Rego Reis
• Grantee: Victoria de Paiva Oliveira
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The project aims to generate immortalized PDAC cell lines resistant to gemcitabine, and the molecular characterization of this resistance. Such lines will be generated by prolonged treatments under sub-lethal concentrations of the drug and will have their resistance evaluated by determining the expression of resistance marker genes already described in the literature by RT-qPCR. In addition to widely studied PDAC lines, a line derived from PDAC xenotransplantation will also be used, which more reliably recapitulates the patient's tumor microenvironment. The results obtained in this project will be the basis for a project under development in the Laboratory of Integrated Research in Cancer of the IQ-USP, which aims to perform the scan of components of the epigenetic machinery functionally associated with chemoresistance and sensitivity to gemcitabine in cancer of pancreas.