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Acquired pellicle engineering to protect against dental erosion: use of a new hand-held optical reflectometer to evaluate the protective role of proteins/peptides

Grant number: 19/08437-5
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 01, 2019
Effective date (End): July 31, 2020
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Marília Afonso Rabelo Buzalaf
Grantee:Vinícius Taioqui Pelá
Supervisor: Adrian Lussi
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Research place: University of Bern, Switzerland  
Associated to the scholarship:17/04857-4 - Acquired pellicle engineering for control of dental erosion: in situ evaluation of protective potential by a new cistatin derived from sugar cane, BP.DR


Recently, studies involving the incorporation of proteins into the acquired pellicle have been proposed as a new strategy for the control of dental erosion. However, probably the greatest challenges in dental erosion research is to find an assessment method that is able analyze dental erosion clinically. Recently, a new hand-held device called Reflectometer Optipen has been developed by Prof. Lussi´s group and showed good results to assess initial erosion in vitro. Due to its characteristics, this device has a great potential to be employed clinically. To date, the device is not commercially available and is only found at Prof. Lussi´s laboratory. Thus, it is quite important that researchers from other groups get training on the use of this device for future clinical application. Based on this, the objective of this study is to evaluate the protective effect of the incorporation of proteins/peptides (CaneCPI-5, Statherin, Hemoglobin) alone or combined, on the prevention of dental erosion in vitro, as evaluated using the Reflectometer Optipen. One hundred specimens of human enamel (molars) will be prepared and divided into five groups (n=20) according with the treatment: 1) deionized water (control), 2) 0.1 mg/ml CaneCPI-5 solution; 3) 1.0 mg/ml hemoglobin solution; 4) 1,88 X 10-5 M Statherin peptide (DpSpSEEKFLRRIGRFG) solution; 5) Combination of all the proteins/peptides (CaneCPI-5, Hemoglobin, Statherin). After prophylaxis, initial surface reflection intensity (SRI) will be measured using the Reflectometer Optipen. The samples will be exposed to the solutions for 2 hours at 37ºC under agitation. Then, the samples will be incubated in stimulated saliva provided by three volunteers, for the formation of the acquired pellicle for 2 hours at 37°C under stirring. For the erosive process, the samples will be incubated in 1% citric acid (pH 3,6) for 1 min at 37°C. Each specimen will be treated once/day over 3 days. After the last erosive challenge, specimens will receive prophylaxis and final SRI will be measured and relative SRI loss will be calculated. Data will be analyzed by appropriate statistical tests, after normality and homogeneity evaluation (p<0.05).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PELA, VINICIUS TAIOQUI; NIEMEYER, SAMIRA HELENA; BAUMANN, TOMMY; LEVY, FLAVIA MAUAD; HENRIQUE-SILVA, FLAVIO; LUSSI, ADRIAN; CARVALHO, THIAGO SAADS; RABELO BUZALAF, MARILIA AFONSO. Acquired Pellicle Engineering Using a Combination of Organic (Sugarcane Cystatin) and Inorganic (Sodium Fluoride) Components against Dental Erosion. Caries Research, v. 56, n. 2, p. 8-pg., . (19/08437-5)
PELA, VINICIUS TAIOQUI; RABELO BUZALAF, MARILIA AFONSO; NIEMEYER, SAMIRA HELENA; BAUMANN, TOMMY; HENRIQUE-SILVA, FLAVIO; TOYAMA, DANYELLE; CRUSCA, EDSON; MARCHETTO, REINALDO; LUSSI, ADRIAN; CARVALHO, THIAGO SAADS. Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface. Journal of Dentistry, v. 107, . (19/08437-5)
PELA, VINICIUS TAIOQUI; VENTURA, TALITA MENDES OLIVEIRA; TAIRA, EVEN AKEMI; THOMASSIAN, LARISSA TERCILIA GRIZZO; BRITO, LEONARDO; MATUHARA, YAN EIJI; HENRIQUE-SILVA, FLAVIO; GROISMAN, SONIA; CARVALHO, THIAGO SAADS; LUSSI, ADRIAN; et al. Use of Reflectometer Optipen to assess the preventive effect of a sugarcane cystatin on initial dental erosion in vivo. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 141, p. 6-pg., . (17/04857-4, 19/26070-1, 19/08437-5, 18/12041-7)

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