Recently, studies involving the incorporation of proteins into the acquired pellicle have been proposed as a new strategy for the control of dental erosion. However, probably the greatest challenges in dental erosion research is to find an assessment method that is able analyze dental erosion clinically. Recently, a new hand-held device called Reflectometer Optipen has been developed by Prof. Lussi´s group and showed good results to assess initial erosion in vitro. Due to its characteristics, this device has a great potential to be employed clinically. To date, the device is not commercially available and is only found at Prof. Lussi´s laboratory. Thus, it is quite important that researchers from other groups get training on the use of this device for future clinical application. Based on this, the objective of this study is to evaluate the protective effect of the incorporation of proteins/peptides (CaneCPI-5, Statherin, Hemoglobin) alone or combined, on the prevention of dental erosion in vitro, as evaluated using the Reflectometer Optipen. One hundred specimens of human enamel (molars) will be prepared and divided into five groups (n=20) according with the treatment: 1) deionized water (control), 2) 0.1 mg/ml CaneCPI-5 solution; 3) 1.0 mg/ml hemoglobin solution; 4) 1,88 X 10-5 M Statherin peptide (DpSpSEEKFLRRIGRFG) solution; 5) Combination of all the proteins/peptides (CaneCPI-5, Hemoglobin, Statherin). After prophylaxis, initial surface reflection intensity (SRI) will be measured using the Reflectometer Optipen. The samples will be exposed to the solutions for 2 hours at 37ºC under agitation. Then, the samples will be incubated in stimulated saliva provided by three volunteers, for the formation of the acquired pellicle for 2 hours at 37°C under stirring. For the erosive process, the samples will be incubated in 1% citric acid (pH 3,6) for 1 min at 37°C. Each specimen will be treated once/day over 3 days. After the last erosive challenge, specimens will receive prophylaxis and final SRI will be measured and relative SRI loss will be calculated. Data will be analyzed by appropriate statistical tests, after normality and homogeneity evaluation (p<0.05).
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