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Study of pomegranate seed oil emulsions and particles gastrointestinal digestion by INFOGEST static in vitro simulation

Grant number: 19/10062-0
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): December 01, 2019
Effective date (End): November 30, 2020
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal researcher:Miriam Dupas Hubinger
Grantee:Talita Aline Comunian
Supervisor abroad: Andre Brodkorb
Home Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Research place: Moorepark Food Research Centre, Ireland  
Associated to the scholarship:18/01710-5 - Influence of the oil-in water emulsion composition on the properties of the structure and functionality of particles obtained by spray drying, BP.PD

Abstract

The oil extracted from the pomegranate seed, a by-product of the juice industry, contains several promoting compounds such as phospholipids, tocopherols, triterpenoids and punicic acid. However, the oil is susceptible to oxidation when in contact to light, oxygen, moisture and heating, making its handling and application to food products difficult. Encapsulation of the bioactive extract can be an alternative to minimize instabilities. Moreover, understanding the digestive behavior of encapsulated compounds is essential to understand and control the release of the oil from the encapsulation system and estimate its bioaccessibility during gastro-intestinal transit. Therefore, the objective of this project is the in vitro digestibility study of pomegranate seed oil encapsulated in oil-in-water emulsions in six matrices: whey protein microgels (WPI), WPI in its natural form and WPI in combination with modified starch (Capsul®) and also these spray dried formulations. The static in vitro digestion INFOGEST method will be used, which simulates gastro-intestinal digestion of food systems considering many factors such as concentrations and types of enzymes and salts, pH and digestion time. For that, lipid analysis will be performed considering total lipid extraction, derivatization of lipid, analysis of fatty acid methyl ester, state of the art microscopy and particle size of the digested samples in the oral, gastric and intestinal phase. The proposed study will produce highly innovative encapsulation vehicles for health-promotion, bioactive compounds that will bring benefits to consumers and society.

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