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Influence of sugarcane bagasse saccharification by laccase for fermentative production of biofuels

Grant number: 18/26470-7
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): June 01, 2019
Effective date (End): May 31, 2021
Field of knowledge:Engineering - Sanitary Engineering
Principal Investigator:Maria Bernadete Amâncio Varesche
Grantee:Camila Abreu Borges da Silva Rabelo
Home Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery, AP.TEM

Abstract

This project aims to apply two differential techniques in the bioconversion of sugarcane bagasse (SCB) in hydrogen, methane and organic acids. The first technique will be the application of the laccase enzyme to the SCB saccharification and the second will be the quantification of the number of copies of hydrogenase gene by quantitative real time Polymerase Chain Reaction (qPCR) and gene expression by reverse transcription and quantitative Real Time Polymerase Chain Reaction (RT-qPCR) during the SCB bioconversion by pure culture. Therefore, we will seek new partnerships with the Laboratory of Functional and Structural Biochemistry of UFSCar and with the Laboratory of Animal Health of Embrapa to make them feasible. From this, the proposed methodology is composed of 5 stages. Step 1 will consist of the laccase extraction from the yeast Pichia pastoris. In step 2, the laccase will be applied as SCB enzymatic pretreatment, and the substrate concentration, pH and temperature factors will be optimized by factorial design. In step 3, the fermentation of hydrolyzed SCB by pure culture of Paraclostridium benzoelyticum will be performed and during this process, the expression of hydrogenase enzymes will be monitored and compared with the metabolites production. In step 4, a study will be carried out to replace the liquid medium of the batch to increase the production of hydrogen. And finally, in step 5, the batch effluents from step 4 will be used to produce methane with the addition of methanogenic inoculum. It is expected with the results obtained in the present study, to present a new viable possibility for hydrolysis of SCB. It is intended to use and make feasible the use of qPCR and RT-qPCR for the monitoring of fermentation processes of biofuels production. These possibilities are still not routinely carried out in the Biological Processes Laboratory, so the partnership with Embrapa and UFSCar will be extremely valuable to aggregate knowledge in new research fronts. (AU)

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