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Application of low temperature plasma at atmospheric pressure in the control of cariogenic biofilms

Grant number: 19/01676-4
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 28, 2019
Effective date (End): September 27, 2020
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Cristiane Yumi Koga Ito
Grantee:Leandro Wagner Figueira
Supervisor abroad: Simone Duarte
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Local de pesquisa : Indiana University, United States  
Associated to the scholarship:18/17707-3 - Application of cold atmospheric plasma for the control of cariogenic biofilms, BP.DR


The aim of this study is to evaluate the application of low temperature plasma atatmospheric pressure (LTAPP) in the control of cariogenic biofilms. A commerciallyavailable plasma-generating equipment (Kinpen®) will be evaluated at IUPUI. Physicalparameters of LTAPP effective against polymicrobial biofilms composed ofcombinations of the species Streptococcus mutans, Streptococcus gordonii,Streptococcus sanguinis, Lactobacillus casei, Candida albicans and Actinomycesviscosus will be established. Multi-species biofilms will be submitted to LTAPP andquantitative and microscopic analyzes (confocal, scanning electron microscopy andfluorescence) will be performed. The action of LTAPP on biofilms formed in situ withdifferent degrees of cariogenicity will also be evaluated. Experimentally induced cariescavities will be submitted to LTAPP, restored and evaluated. Negative control (notreatment) and positive control (chlorhexidine 2%) will be included. The cellular effectsof LTAPP on S. mutans, L. casei and A. viscosus will be studied by scanning electronmicroscopy and Fourier transform infrared (FT-IR) spectroscopy. Finally, thecytotoxicity of LTAPP in the effective conditions on gingival fibroblasts and primaryculture of fibroblasts of the dental pulp will be evaluated. All assays will be performed intriplicate in three independent experiments. The results will be analyzed for distribution.The most appropriate statistical tests will then be selected. The level of significance willbe 5%