Comparative analysis of lymphocytic infiltrate in oral leucoplastic lesions and oral lichen planus

• Grant number: 18/22236-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2019
• End date: December 31, 2019
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Andreia Bufalino
• Grantee: Mariana Paravani Palaçon
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Squamous cell carcinoma (SCC) accounts for more than 95% of all malignancies involving the oral cavity, and often these tumors are preceded by clinical alterations that have a clear potential for malignant transformation, which are denominated oral potentially malignant disorders (OPMDs). Among these, proliferative verrucous leukoplakia (PVL) is characterized by multiple white plaques that present a persistent and progressive clinical behavior for malignancy, with malignant transformation rates greater than 70% and an inadequate response to all treatment modalities. Recent studies have described that PVL, particularly in the early stages of the disease, can microscopically reveal an inflammatory infiltrate with a predominance of T-lymphocytes in the lamina propria with absence of dysplasia, similar to the histopathological findings found in oral lichen planus (OLP), compromising early diagnosis of PVL. In addition, preliminary data obtained by our group suggest that the inflammatory infiltrate associated with PVL is related to the etiology and/or aggressive clinical behavior of this OPMD. Thus, this study aims to perform a comparative analysis between OLP and PVL samples: (1) evaluating the density of the helper, cytotoxic and B-cell lymphocytes in the intraepithelial and conjunctival LPO samples and in the LVP; and (2) to assess the activation status of the helper and cytotoxic T lymphocyte subtypes in OLP and LVP.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, DARCY; BARBEIRO, CAMILA DE OLIVEIRA; PALACON, MARIANA PARAVANI; BIANCARDI, MARIEL RUIVO; FERRISSE, TULIO MORANDIN; SILVEIRA, HEITOR ALBERGONI; CASTILHO, ROGERIO MORAES; DE ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE; BUFALINO, ANDREIA. High density of CD8 T cell and immune imbalance of T lymphocytes subsets are associated with proliferative verrucous leukoplakia. IMMUNOLOGY, v. 168, n. 1, p. 14-pg., 2022-09-03. (17/01798-7, 17/01438-0, 13/07276-1, 18/22236-0, 21/01191-0, 18/04954-2, 17/17288-8)

PALACON, MARIANA PARAVANI; BARBEIRO, CAMILA DE OLIVEIRA; FERNANDES, DARCY; BIANCARDI, MARIEL RUIVO; SILVEIRA, HEITOR ALBERGONI; FERRISSE, TULIO MORANDIN; LEON, JORGE ESQUICHE; KUJAN, OMAR; BUFALINO, ANDREIA. Macrophages CD163+and Factor XIIIa plus Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 6, p. 12-pg., 2023-03-01. (18/04954-2, 18/22236-0, 13/07276-1, 21/01191-0, 17/01798-7, 17/17288-8)

BARBEIRO, CAMILA DE OLIVEIRA; FERNANDES, DARCY; PALACON, MARIANA PARAVANI; CASTILHO, ROGERIO MORAES; DE ALMEIDA, LUCIANA YAMAMOTO; BUFALINO, ANDREIA. Inflammatory Cells Can Alter the Levels of H3K9ac and gamma H2AX in Dysplastic Cells and Favor Tumor Phenotype. JOURNAL OF PERSONALIZED MEDICINE, v. 13, n. 4, p. 15-pg., 2023-04-01. (18/04954-2, 22/14672-0, 18/22236-0, 17/01798-7, 17/01438-0)