Influence of silver nanoparticles (AgNPs) on the in vitro neuromuscular blockade of Philodryas olfersii (Dipsadidae) snake venom

• Grant number: 19/05891-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: July 31, 2020
• Field of knowledge: Biological Sciences - Pharmacology - Autonomic Pharmacology
• Principal Investigator: Yoko Oshima Franco
• Grantee: Anna Paula Farias de França
• Host Institution: Pró-Reitoria de Pós-Graduação, Pesquisa, Extensão e Inovação. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil

Abstract

Silver nanoparticles (AgNPs) have antibacterial, antifungal and antiviral characteristics, with numerous applications in medicine. On the other hand, the envenoming caused by Philodryas olfersii (Dipsadidade) snake induce edema (swelling), erythema and bruises (dark spots), regional lymphadenopathy (lymphonodes dilation), neurotoxic and myotoxic effects. The symptomatology of this poisoning is similar to that seen with bothropic ("jararaca") envenoming, by the presence of similar biological constituents. Epidemiologically the incidence of accidents caused by P. olfersii is low due to anatomy of teeth, since there are opisthoglyphic snakes, i.e., they have the inoculator teeth at the bottom of the mouth, in the posterior portion of the maxilla, hindering the venom inoculation. The venom of P. olfersii induce an in vitro neuromuscular blockade in mouse and chick preparations, being more sensitive in avian than in mouse. For this reason, in this project, silver nanoparticles will be tested on the neurotoxic effect in vitro of Philodryas olfersii snake venom, in chick biventer cervicis preparations.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PROENCA-ASSUNCAO, JAQUELINE DE CASSIA; CONSTANTINO, EDERSON; FARIAS-DE-FRANCA, ANNA PAULA; NOGUEIRA, FLAVIA APARECIDA RESENDE; CONSONNI, SILVIO ROBERTO; CHAUD, MARCO VINICIUS; DOS SANTOS, CAROLINA ALVES; OSHIMA-FRANCO, YOKO. Mutagenicity of silver nanoparticles synthesized with curcumin (Cur-AgNPs). JOURNAL OF SAUDI CHEMICAL SOCIETY, v. 25, n. 9, SEP 2021. (19/05891-7, 12/08271-0)

PROENCA-ASSUNCAO, JAQUELINE DE CASSIA; FARIAS-DE-FRANCA, ANNA PAULA; TRIBUIANI, NATALIA; COGO, JOSE CARLOS; COLLACO, RITA DE CASSIA; RANDAZZO-MOURA, PRISCILA; CONSONNI, SILVIO ROBERTO; CHAUD, MARCO VINICIUS; DOS SANTOS, CAROLINA ALVES; OSHIMA-FRANCO, YOKO. The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 16, p. 3555-3564, 2021. (12/08271-0, 19/05891-7)