Trans-resveratrol solid dispersions incorporated into polyvinylpyrrolidone membranes for cutaneous application: development, characterization and biological evaluation in vitro and in vivo

• Grant number: 19/00164-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2019
• End date: November 30, 2021
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Marlus Chorilli
• Grantee: Bruno Vincenzo Fiod Riccio
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Cutaneous inflammation is related to the inflammatory mediators synthesis, when produced in exacerbated quantities and in its chronic form, they can lead to skin diseases such as atopic dermatitis. Trans-resveratrol (TR) is a non-flavonoid polyphenol from the class of stilbenes obtained from vegetal sources such as grapes, and it is shown to be effective as an anti-inflammatory agent due to the reduction or inhibition of mediators such as histamine, cytokines and cyclooxygenase-2 (COX-2), but its low solubility in water becomes a limiting factor for their incorporation into pharmaceutical forms. The development of solid dispersions (DS) of TR in chitosan (QS) containing Poloxamer® 407 (P407), TPGS and Aersosil® arises with an innovative strategy to improve the solubility of TR. In order to make DS administration viable, a use of polyvinylpyrrolidone (PVP) films with bioadhesive properties may be of interest, since these systems allow a longer local retention time of the drug. The present study aims to develop, characterize and evaluate an in vitro cytotoxicity and in vivo anti-inflammatory activity of TR DS incorporated into PVP membranes for dermal administration. The DS were obtained by the solvent casting method and characterized by granulometric analysis, solubility study, differential scanning calorimetry (DSC), X-ray diffraction (XRD), scanning electron microscopy (SEM-FEG) and in vitro dissolution. Subsequently, the DS incorporated in PVP membranes are capable of measuring the intensity, X-ray intensity, heat transfer rays, differential scanning calorimetry, X-ray diffraction, infrared spectroscopy with Fourier (FTIR) and field emission scanning electron microscopy. Release and in vitro skin emission assays are performed for DS and free and drug-associated membranes. Biological and in vivo assay to evaluate the anti-inflammatory effect of formulations on animals of ear edema induced by chroton oil. Therefore, it is expected an innovative selection that allows the delivery of a way to make its use viable as an anti-inflammatory in cutaneous diseases. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RICCIO, BRUNO V. F.; SPOSITO, LARISSA; CARVALHO, GABRIELA C.; FERRARI, PRISCILEILA C.; CHORILLI, MARLUS. Resveratrol isoforms and conjugates: A review from biosynthesis in plants to elimination from the human body. ARCHIV DER PHARMAZIE, v. 353, n. 12, SEP 2020. (19/00164-0)

RICCIO, BRUNO VINCENZO FIOD; DO NASCIMENTO, ANDRE LUIZ CARNEIRO SOARES; MENEGUIN, ANDREIA BAGLIOTTI; RODERO, CAMILA FERNANDA; SANTOS, KAIO PINI; SABIO, RAFAEL MIGUEL; DE ANNUNZIO, SARAH RAQUEL; FONTANA, CARLA RAQUEL; BARUD, HERNANE DA SILVA; FERRARI, PRISCILEILA COLERATO; et al. Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation. PHARMACEUTICS, v. 14, n. 6, p. 21-pg., 2022-06-01. (19/19817-3, 18/09088-1, 18/23015-7, 18/25377-3, 18/23357-5, 19/00164-0)

FIOD RICCIO, BRUNO VINCENZO; POLLI SILVESTRE, AMANDA LETICIA; MENEGUIN, ANDREIA BAGLIOTTI; RIBEIRO, TAIS DE CASSIA; KLOSOWSKI, ANA BEATRIZ; COLERATO FERRARI, PRISCILEILA; CHORILLI, MARLUS. Exploiting Polymeric Films as a Multipurpose Drug Delivery System: a Review. AAPS PHARMSCITECH, v. 23, n. 7, p. 23-pg., 2022-09-28. (19/00164-0)

RICCIO, BRUNO VINCENZO FIOD; MENEGUIN, ANDREIA BAGLIOTTI; BAVELONI, FRANCIELE GARCIA; DE ANTONI, JULIANA AENISHANSLIN; ROBUSTI, LEDA MARIA GORLA; GREMIAO, MARIA PALMIRA DAFLON; FERRARI, PRISCILEILA COLERATO; CHORILLI, MARLUS. Biopharmaceutical and nanotoxicological aspects of cyclodextrins for non-invasive topical treatments: A critical review. JOURNAL OF APPLIED TOXICOLOGY, v. N/A, p. 11-pg., 2023-01-22. (19/00164-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RICCIO, Bruno Vincenzo Fiod. Trans-resveratrol solid dispersions incorporated into polyvinylpyrrolidone membranes for cutaneous administration: development, characterization and in vitro and in vivo biological evaluation. 2021. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.