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Formulation of an optimized synergistic enzyme cocktail with accessory enzymes to enhances the hydrolytic performance of Panicum Maximum Jacq

Grant number: 19/07141-5
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 01, 2019
Effective date (End): February 29, 2020
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Principal researcher:Maria de Lourdes Teixeira de Moraes Polizeli
Grantee:Emanuelle Neiverth de Freitas
Supervisor abroad: John Nicholas Saddler
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Research place: University of British Columbia (UBC), Canada  
Associated to the scholarship:17/23989-9 - Lignocellulosic biomass of Panicum maximum Jacq. forage grass produced in simulated future climate conditions: potential source of fermentable sugars for bioenergy, BP.DR

Abstract

The depletion of fossil fuels and the pollution generated by them, such as the emission of greenhouse gases, has stimulated the search for alternative and renewable sources of energy. Biomass produced by fast-growing grasses such as Panicum genus, commonly used as fodder, can be important sources of clean energy production, but it is necessary to estimate their biomass potential production in simulated future climate situations. Lignocellulosic biomass is mainly composed of cellulose, hemicellulose and lignin, and therefore, it is necessary the joint action of many enzymes for fermentable sugars production. However, the cellulolytic core of enzymes cannot degrade biomass alone. Instead, to achieve effective lignocellulose deconstruction, the enzyme mixture must also contain accessory enzymes/proteins such as hemicellulases, lytic polysaccharide monooxygenases, and non-hydrolytic/non-oxidative proteins, as expansins. These enzymes act through the opening up of the lignocellulosic matrix and appear to act synergistically with the canonical hydrolytic enzyme facilitating biomass degradation. Some studies have been investigated the addition of accessory enzymes in enzymes cocktail and showed a significantly increase in hydrolysis yield when these enzymes are in the cocktail formulation. It is known that climate conditions can affect plant physiology and modify plant lignocellulosic composition, synthesis of cell wall components, which may indirectly affect cellulose synthesis. Thus, the aim of this work is to develop a specific enzymatic cocktail to each future climate condition (elevated temperature, elevated CO2 concentrations and combined effects) that leads to greater hydrolysis efficiency, verifying the influence of the conditions on the composition and profitability of each grass individually. Furthermore, it will be analyzed if the addition of accessory enzymes will lead to an improvement of saccharification process, as well it will be investigated the action of the cocktail enzymes over the fiber and cellulose structure. For this purpose, it will be applied some techniques as Fiber Quality Assay (FQA), Settleablity test, Carbohydrate Binding Modules tagged with fluorescence probe and X ray Diffraction.

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