Prostatic telocytes, smooth muscle cells, myofibroblasts and fibroblasts: origin, interrelationships and transdifferentiation.

• Grant number: 18/08945-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date until: July 01, 2019
• End date until: December 31, 2022
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Hernandes Faustino de Carvalho
• Grantee: Bruno Domingos Azevedo Sanches
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 21/12210-6 - Investigation of telocyte vesicles as a tool for tissue regeneration and evaluation of telocyte progenitor capacity, BE.EP.PD

Abstract

The origin of myofibroblasts and telocytes in the prostate is still unclear. It also unknown whether a single mesenchymal progenitor cell capable of giving rise to fibroblasts, smooth muscle cells, myofibroblasts and telocytes exist. In addition, little is known about the interrelationships among these cell types of the prostate stroma. Telocytes are CD34-positive interstitial cells, which were characterized in the prostatic stroma and in several other organs. It was thought that these cells could originate the myofibroblasts, fibroblasts and smooth muscle cells, but there is no evidence to prove these ideas. The exact function of these cells in the prostate is still elusive. The present project aims at investigating the existence of a common mesenchymal progenitor for smooth muscle cells, myofibroblasts, telocytes and prostatic fibroblasts, and the possible interrelationships among these cell types, in ex vivo and in vitro experiments. A second objective is to evaluate a possible supportive role of prostatic telocytes in the differentiation of smooth muscle cells. The experimental data will be important for a better understanding of progeny relationships among smooth muscle cells, fibroblasts and myofibroblasts, as well as for sheding light on a network of interrelationships between these cell types. Finally, the data might suggest new therapeutic targets for tissue regeneration, which is important for the treatment of degenerative diseases of the smooth muscle, as well as to target the formation of myofibroblasts, which are associated with fibrosis and even cancer.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANCHES, BRUNO D. A.; MALDARINE, JULIANA D. S.; TAMARINDO, GUILHERME H.; DA SILVA, ALANA D. T.; LIMA, MARIA L. D.; RAHAL, PAULA; GOES, REJANE M.; TABOGA, SEBASTIAO R.; CARVALHO, HERNANDES F.. Explant culture: A relevant tool for the study of telocytes. Cell Biology International, v. 44, n. 12, AUG 2020. (18/08945-8)

SANCHES, BRUNO D. A.; LEONEL, ELLEN C. R.; MALDARINE, JULIANA S.; TAMARINDO, GUILHERME H.; BARQUILHA, CAROLINE N.; FELISBINO, SERGIO L.; GOES, REJANE M.; VILAMAIOR, PATRICIA S. L.; TABOGA, SEBASTIAO R.. Telocytes are associated with tissue remodeling and angiogenesis during the postlactational involution of the mammary gland in gerbils. Cell Biology International, v. 44, n. 12, SEP 2020. (18/23383-6, 18/08945-8)

MALDARINE, JULIANA S.; SANCHES, BRUNO D. A.; SANTOS, VITORIA A.; GOES, REJANE M.; VILAMAIOR, PATRICIA S. L.; CARVALHO, HERNANDES F.; TABOGA, SEBASTIAO R.. The complex role of telocytes in female prostate tumorigenesis in a rodent model. Cell Biology International, v. N/A, p. 15-pg., 2022-05-21. (18/08945-8, 19/14201-4, 18/23383-6)

SANCHES, BRUNO DOMINGOS AZEVEDO; TAMARINDO, GUILHERME HENRIQUE; DA SILVA, ALANA DELLA TORRE; AMARO, GUSTAVO MATHEUS; DOS SANTOS MALDARINE, JULIANA; DOS SANTOS, VITORIA ALARIO; GUERRA, LUIZ HENRIQUE ALVES; BARALDI, CAROLINA MARQUES BEDOLO; GOES, REJANE MAIRA; TABOGA, SEBASTIAO ROBERTO; et al. Stromal cell-derived factor 1 (SDF-1) increases the number of telocytes in ex vivo and in vitro assays. Histochemistry and Cell Biology, v. N/A, p. 15-pg., 2023-07-20. (21/02303-7, 18/08945-8)

SANCHES, BRUNO D. A.; MALDARINE, JULIANA S.; VILAMAIOR, PATRICIA S. L.; FELISBINO, SERGIO L.; CARVALHO, HERNANDES F.; TABOGA, SEBASTIAO R.. Stromal cell interplay in prostate development, physiology, and pathological conditions. PROSTATE, v. 81, n. 13, JUL 2021. (18/08945-8, 18/23383-6)

SANCHES, BRUNO DOMINGOS AZEVEDO; TAMARINDO, GUILHERME HENRIQUE; MALDARINE, JULIANA DOS SANTOS; DA SILVA, ALANA DELLA TORRE; DOS SANTOS, VITORIA ALARIO; GOES, REJANE MAIRA; TABOGA, SEBASTIAO ROBERTO; CARVALHO, HERNANDES F.. Telocytes of the male urogenital system: Interrelationships, possible functions, and pathological implications. Cell Biology International, v. 45, n. 8, p. 1613-1623, AUG 2021. (18/08945-8)

AZEVEDO SANCHES, BRUNO DOMINGOS; TAMARINDO, GUILHERME HENRIQUE; MALDARINE, JULIANA DOS SANTOS; DA SILVA, ALANA DELLA TORRE; DOS SANTOS, VITORIA ALARIO; DUARTE LIMA, MARIA LETICIA; RAHAL, PAULA; GOES, REJANE MAIRA; TABOGA, SEBASTIAO ROBERTO; FELISBINO, SERGIO LUIS; et al. Telocytes contribute to aging-related modifications in the prostate. SCIENTIFIC REPORTS, v. 10, n. 1, DEC 7 2020. (18/08945-8)