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Optimal essential amino acids ratio for pacu in the termination phase by the deletion method

Grant number: 19/06343-3
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2019
Effective date (End): September 30, 2020
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:João Batista Kochenborger Fernandes
Grantee:Thaís da Silva Oliveira
Home Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated research grant:13/25761-4 - Modeling production and nutritional requirements of poultry and fish, AP.TEM

Abstract

Among the techniques to determine nutritional requirement and ideal ratios among essential amino acids for fish, the purpose of the deletion method is to determine the ratios among the essential amino acids in a single experiment, maintaining stable all the variables related to the environment, lot of fish and diets. In addition, it is possible to estimate the amino acid requirement based on the ideal protein concept. The aim of this study will be to determine the optimal essential amino acids ratio, to meet the amino acid requirement of pacus in the termination phase, by the deletion method. Thus, 165 fish with a mean initial weight of 1.0 kg will be distributed in 33 tanks (1000L) in a water recirculation system, equipped with mechanical and biological filtration, heat exchanger and aeration. The experiment will last 60 days and will consist of 11 treatments diets with three replications, distributed in a completely randomized design. One of the experimental diets will be the control diet and the other diets will present the deletion of 45% of the essential amino acids to be tested. The determination of the requirements of essential amino acid will be performed by the ratio between the nitrogen retention by pacu and the amount of the amino acid deleted in the evaluated diet. The obtained data will be submitted to analysis of variance (One-way ANOVA), by the statistical program SAS, 2008. If there is a significant difference, Tukey's test will be applied (p <0.05). The values found will be submitted to the Dunnet test (p <0.05), to compare the treatments with the positive control.