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Impact of long-term application of lime and gypsum on tropical no-tillage system on soil microbiome changes and NITROGEN-CYCLE

Grant number: 19/12764-1
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): December 01, 2019
Effective date (End): September 30, 2020
Field of knowledge:Agronomical Sciences - Agronomy - Soil Science
Principal Investigator:Carlos Alexandre Costa Crusciol
Grantee:João William Bossolani
Supervisor abroad: Eiko Eurya Kuramae
Home Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Local de pesquisa : Royal Netherlands Academy of Arts and Sciences, Netherlands  
Associated to the scholarship:18/11063-7 - Lime and gypsum in long-term experiment in no-tillage system: quality of soil and recovery of 15N-fertilizer by crops, BP.DR


The liming affects biological activities in the soil, and can to influence both the physical and chemistry of the soil and, thereby, changes crop growth and yields. Additionally, to use de lime, the gypsum use as a complementary practice can influence on root system development and resource availability. This changes the soil capacity to assembly the bacterial community and as to influence the nitrogen cycling potential of soils. Our research project is part of a long-term experiment, installed in 2002/2003, at the College of Agricultural Sciences, UNESP, Botucatu (SP-Brazil). Since its deployment, the activity and microbial communities in relation to soil N cycle have never been evaluated. Thus, following the experiment, which should probably be the only one in the State of São Paulo with more than 17 years of evaluation, our aim is to investigate if and how lime and/or gypsum change diversity, taxonomic profile, and structure of soil microbial communities and decipher the microbial process (es) related to the nitrogen cycle. The experimental design will be in randomized blocks with 4 replicates. The treatments are 1: without application of lime and gypsum (Control); 2: Lime application (13,040 kg ha-1); 3: Gypsum application (10,000 kg ha-1); 4: Lime and Gypsum application. The bulk soil and rhizospheric soil of maize will be to evaluate. The chemical properties of soil will be analyzed and associate with the microbiome of soil.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BOSSOLANI, JOAO WILLIAM; COSTA CRUSCIOL, CARLOS ALEXANDRE; MERLOTI, LUIS FERNANDO; MORETTI, LUIZ GUSTAVO; COSTA, NIDIA RAQUEL; TSAI, SIU MUI; KURAMAE, EIKO EURYA. Long-term lime and gypsum amendment increase nitrogen fixation and decrease nitrification and denitrification gene abundances in the rhizosphere and soil in a tropical no-till intercropping system. Geoderma, v. 375, OCT 1 2020. Web of Science Citations: 0.

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