Different types of ozone administration in the inactivation of Pythium insidiosum - in vitro study

• Grant number: 19/13812-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: September 30, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Jair Camargo Ferreira
• Grantee: Francine Gabriela Guiotto
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

The goal of the present study is to evaluate the inactivation power of three presentation forms of ozone (O3) on Pythium insidiosum isolated from horses. Pre-cultured aliquots of the pathogen will be divided into six (6) groups according to the treatment: O3-H2O, O3-Gas, O3-Oil, Control-H2O, Control-Gas and Control-Oil (n = 5 aliquots /group). In the O3-H2O group, the aliquots will be immersed in 20 mL of ozonated distilled water (69 ¼g O3 mL-1) for 15 minutes. In the O3-Gas group, the aliquots will be exposed to an O3-rich atmosphere (69 ¼g O3 mL-1) for 15 minutes. In the O3-Oil group, the aliquots will be immersed in 20 mL of O3-enriched sunflower oil for 15 minutes. Control-H2O, Control-Gas and Control-Oil groups will be treated respectively with pure distilled water, oxygen gas and non-ozonated sunflower oil. The volumes and the times of exposure of each control treatment will be similar to described for the respective presentation form of presentation in the treated group. Immediately after each treatment, the aliquots will be incubated at 35°C for four weeks. The parameters to be evaluated during the post-treatment period are: a) Inhibition of growth rate, b) Cell wall damage, c) germicidal/germistatic action of O3, and d) changes in the superficial architecture of P. insidiosum.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, JAIR CAMARGO; PIRES, REGINA HELENA; DA COSTA, GUILHERME BARBOSA; CARRIJO, BRUNA NONATO; GUIOTTO, FRANCINE GABRIELA; RODRIGUES, VIVIANI SILVA. The In Vitro Effect of Ozone Therapy Against Equine Pythium insidiosum. Journal of Equine Veterinary Science, v. 98, MAR 2021. (19/13812-0, 19/15532-4)

CARRIJO, BRUNA N.; PIRES, REGINA H.; COSTA, GUILHERME B.; GUIOTTO, FRANCINE G.; RODRIGUES, VIVIANI S.; FERREIRA, JAIR C.. Ozone Gas and Ozonized Sunflower Oil as Alternative Therapies against Pythium Insidiosum Isolated from Dogs. OZONE-SCIENCE & ENGINEERING, JUN 2021. (19/13812-0, 19/15532-4)

CARRIJO, BRUNA N.; PIRES, REGINA H.; COSTA, GUILHERME B.; GUIOTTO, FRANCINE G.; RODRIGUES, VIVIANI S.; FERREIRA, JAIR C.. Ozone Gas and Ozonized Sunflower Oil as Alternative Therapies against Pythium Insidiosum Isolated from Dogs. OZONE-SCIENCE & ENGINEERING, v. 44, n. 4, p. 9-pg., 2021-06-13. (19/15532-4, 19/13812-0)