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Relationship between milk synthesis, stress and expression of HSPs (heat chock proteins) in the mammary gland of Saanen goats

Grant number: 19/11855-3
Support type:Scholarships abroad - Research
Effective date (Start): September 01, 2019
Effective date (End): June 30, 2020
Field of knowledge:Agronomical Sciences - Animal Husbandry
Principal Investigator:João Alberto Negrão
Grantee:João Alberto Negrão
Host: Geoffrey e Dahl
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Local de pesquisa : University of Florida, Gainesville (UF), United States  


At the end of 2018, the project "Dairy production in Saanen goats: relationships between milk synthesis, stress, apoptosis and gene expression in the mammary gland" was submitted and approved by FAPESP (Process 2018 / 09093-5). This project aims to study how cortisol (CORT, a universal indicator of stress) and heat chock proteins (HSPs), modulate the interaction between insulin (INS), prolactin (PRL), growth hormone (GH) and insulin-like growth factor 1 (IGF-1) and its receptors on breast tissue. In this context, the opportunity arose to work in collaboration with Prof. Dr. Geoffrey E. Dahl (Department of Animal Science, University of Florida, USA) that has an excellent curriculum. Prof. Dahk also coordinates one of the best and most influential research groups in the Lactation Physiology, mainly in epigenetic studies related to gene expression in the mammary gland in animals submitted to heat stress. During this stage in research, I will have the opportunity to improve and apprehend methods and tools such as: the isolation of mammary epithelial cells by cell sorting; the analysis of the transcriptome expression profile of microRNAs and messenger RNAs (mRNA) by last generation sequencing (RNA-Seq); validation of microRNA / target interactions and quantification of the products generated through experiments of overexpression and inhibition of microRNAs in mammary epithelial cells to be implanted in the FZEA / USP Laboratory of Animal Physiology.