Methods that allow the rapid screening of potential drugs against protein targets are highly sought after. Traditional approaches employ a specific biological/biochemical assay to monitor the activity of (usually) pure chemicals from large compound collection libraries. Current techniques that allow mixtures of compounds to be screened individually for binding to protein targets are scarce, and often of limited utility. Oldham group have demonstrated that in-line liquid chromatography-native mass spectrometry has the potential to provide a generic platform to identify bioactive compounds for therapeutic targets. Native ESI-MS is an existing, specialist, technique able to detect non-covalent complexes between proteins and ligands. In this context, the LC-native MS platform will be used for screening binders to protein FKBP and acetylcholinesterase from crude microbial and plant sources.
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