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Monitoring and molecular characterization of arboviros isolated from dippers of the Brazilian west Amazon (State of Rondônia)

Grant number: 18/18867-4
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2019
Effective date (End): August 31, 2020
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Edison Luiz Durigon
Grantee:Cairo Monteiro de Oliveira
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Brazil is a country that holds a large part of its territory taken by tropical forests and other natural ecosystems that allow it ideal conditions the presence of Arbovirus. A number of Arboviruses have already been detected and isolated throughout the territory with many still endemic such as Dengue virus (DENV), Zika virus (ZKV), Chikungunya virus (CHIKV) and many others circulating in nature through reservoir hosts and arthropod vectors which often cause disease outbreaks in animals and humans. The state of Rondônia located in the north of the country has T of its territory covered by the Amazon forest rich in biodiversity of plants and animals. The vegetation, hydrography and equatorial climate create ecosystems with conditions that favor the existence of different species of Arthropods. In this context, the presence of these vectors together with the increasing rate of deforestation and the precarious conditions of basic sanitation of a large part of the population allow potential transmission and establishment of arboviruses in this region. Thus, the present work will aim to monitor the presence of Arbovirus of medical importance such as Flavivirus, Alphavirus and Orthobunyavirus, through the RT-PCR and Nested PCR reaction in the arthropod vectors that circulate in the region of the state of Rondônia, Brazil; as well as the molecular characterization by means of the nucleotide sequencing of the possible Arboviruses found in order to promote information about the circulating viruses and the ability of the vectors in the transmission of certain strains, so that preventive measures can be adopted to combat the vector once that the main form of epidemic prevention focuses on the control of these insects. (AU)