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Optimization of the aromatic compounds pathway in Escherichia coli for phenazines production

Grant number: 19/09332-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2019
Effective date (End): October 31, 2020
Field of knowledge:Engineering - Chemical Engineering
Principal Investigator:Adilson José da Silva
Grantee:Daniel Lossa Altmann
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

Microbial secondary metabolites are recognized as a valuable source of molecules suitable for diverse biotechnological applications. Among them there is a class of natural pigments called phenazines, which can be used fo biopesticides production, biosensors construction, drug development, and more. These molecules are composed by nitrogenous aromatic rings and their biosynthesis occurs from chorismate, which is the last product from the shikimate pathway. Metabolic engineering strategies have been developed to optimize the production of aromatic compounds by this pathway, and this project proposes the application of one of those strategies for the heterologous production of phenazine-1-carboxylic acid (PCA) by recombinant E. coli cells. Specifically, the main goal of this project is to overexpress two enzymes related to the formation of key precursors for the shikimate pathway and to evaluate the PCA production by the modified cells. By using this approach, it is expected that the carbon flow through the aromatics pathway can be increased, favoring the production of PCA by the recombinant E. coli cells.