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Comparison of healing processes in incisional hernia of newly weaned, adult and old rats

Grant number: 19/10347-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2019
Effective date (End): February 28, 2021
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Ana Cristina Aoun Tannuri
Grantee:Raphael Nogueira do Amaral
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Incisional hernia is a condition resulting from inecient healing or inadequate suturing following surgery in the abdomen region, with an opening in the abdominal wall leading to the exit of internal structures (such as organs or tissues) out of the wall. In the pediatric population, several surgeries may lead to the formation of an incisional hernia, with emphasis on the following: stomatal closure and piloromyotomy. It is known that, in general, children have greater adaptive capacity than adults, and healing in pediatric surgeries is generally more ecient. Thus, it is possible to notice a signicant statistical difference in the prevalence of incisional hernia in adults and in children, since in the pediatric population the occurrence is considerably smaller. However, although the incidence of incisional hernia is known to a great extent among the different ages, few studies have shown the differences in the healing process among different age groups who suffer from this hernia. Thus, the goal of this work is to understand and compare how the healing process of the incisional hernia occurs in young, adult and old rats. Twenty-four young Wistar rats (21 days old), 26 adult rats and 26 old rats were divided into groups: J1 (young rats submitted to incisional hernia with vertical cut), J2 (incisional hernia with transverse section), A1 adult rats submitted to incisional hernia provoked with vertical cut), A2 (incisional hernia with transversal cut), V1 (old rats submitted to incisional hernia provoked with vertical cut), V2 (incisional hernia caused with transversal cut) and control. The animals will be weighed daily, cut lengths (before and after euthanasia), histomorphometric, immunohistochemical and molecular studies to compare healing mechanisms in the different groups.