Rosa centifolia L. GLYCOLIC EXTRACT : ANTI-INFLAMMATORY AND ANTIBIOFILM ACTIVITY ON ANAEROBIC BACTERIA OF DENTAL INTEREST

• Grant number: 19/16543-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2019
• End date: March 31, 2022
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luciane Dias de Oliveira
• Grantee: Caroline Maria Ferreira
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

In recent years, there has been a significant increase about research in the worldliterature related to alternative therapies using natural products. These new substances ofvegetable origin have shown promising results for biocompatibility. The objectives of this studyare to analyze in vitro: a) antimicrobial and antibiofilm activity of R. centifolia L. glycolic extracton planktonic cultures and biofilms (monotypic) of Fusobacterium nucleatum e Porphyromonasgingivalis with differents contact times; b) cytotoxicity in macrophages (RAW 264.7); and c) antiinflammatory action of R. centifolia L. extract in macrophages (RAW 264.7) stimulated bylipopolysaccharide (LPS) of Escherichia coli. For verification of antimicrobial activity of theextract, the microdilution broth method will be use, according to the Clinical and LaboratoryStandards Institute (CLSI) M11-A7 on anaerobic microorganisms. The most effectiveconcentration will be analyzed on biofilms for 5 min and 24 h. Subsequently, the cytotoxicity ofthe extract for RAW 264.7 will be evaluated with MTT test. The anti-inflammatory action of theextract will be verified by ELISA, with quantification of pro-inflammatory cytokines (IL-1² andTNF-±), after exposure of RAW 264.7 to the most effective concentrations of the extract, in theabsence and presence of LPS. The results will be statistically analyzed by ANOVA and Tukey Test(p d 0.05).