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The role of xyloglucan in the cell wall architecture determination in sugarcane

Grant number: 19/13936-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): October 01, 2019
Effective date (End): September 30, 2021
Field of knowledge:Biological Sciences - Botany - Pant Physiology
Cooperation agreement: CNPq - INCTs
Principal Investigator:Marcos Silveira Buckeridge
Grantee:Adriana Grandis
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol, AP.TEM

Abstract

Xyloglucan (XG) is the principal hemicellulose in plants, being probably the most abundant polymer in nature after cellulose. XG displays specific interaction with cellulose and is thought to form a covering layer onto cellulose microfibrils, composing a network of polymers that, on one hand alters the mechanical properties of the cell wall and on the other hand protects cell walls against the attack of pathogens. In spite of the fact that XG in grasses are in relatively lower proportion in comparison to eudicotyledons, its function in the interaction with and organization of cellulose in the wall is thought to be still present. The XG-cellulose interaction is likely to be quite important for the development of 2nd generation bioenergy production once this is probably one of the features that determine recalcitrance of biomass to hydrolysis. This proposal aims at understanding the properties of the binding between xyloglucan and cellulose from sugarcane cell walls. Using previously obtained data about sugarcane cell wall composition, this project intends to 1) characterize the fine structure and polysaccharide properties of xyloglucans from sugarcane culms 2) perform binding assays using isolated sugarcane xyloglucan on the surface of different types of cellulose; 3) characterize the binding of xyloglucan specific antibodies to sugarcane xyloglucan isolated and in the walls and use them to develop methods of xyloglucan localization in the walls and tissues. With this data in hands, it will be possible to design strategies to look to the XG-Cellulose interaction in more detail, using different types of microscopy. The perspective of this work is to open the way to cellulose microfibrils surface so that more efficient hydrolysis will be possible and also set the stage up for designing novel biomaterials from sugarcane to be used in other sectors of industry such as paper, cosmetics and food.