Effects of bioactive coffee compounds on human colorectal cancer stem cell-like in spheroids (3D) culture

• Grant number: 19/21413-8
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): November 18, 2019
• Effective date (End): November 17, 2020
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Luís Fernando Barbisan
• Grantee: Ariane Rocha Bartolomeu
• Supervisor abroad: Maria Angel Garcia Chaves
• Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Local de pesquisa : Universidad de Granada (UGR), Spain
• Associated to the scholarship: 17/26217-7 - Action of coffee bioactive compounds towards modulation of miRNAs expression during the colon carcinogenesis in vitro and in vivo, BP.DR

Abstract

Colorectal cancer (CRC) is one of most recurrent cancer worldwide within high mortality rates. Moreover, available cytotoxic options in cancer therapy show low response rates and modest impact on survival, mainly due primary or acquired resistance to conventional treatments as result of cancer stem cell-like exposition. Besides the in vitro studies demonstrate antiproliferative, antioxidant and proapoptotic effects of coffee brew or major bioavailable coffee compounds individually, the literature lack complexity of the compounds interaction observed in whole coffee beverages. In addition, most of these studies remains focused on monolayer in vitro models, lacking the complex enrichment of CRC microenvironment. Nonetheless, the direct contact of colonic epithelium with common coffee bioactive compounds, such as, chlorogenic acid heavily metabolized by colonic microbiota, originates many others bioactive compounds that probably have in situ direct effects. The indicated ongoing project is concerning to reproduce in vitro in a microenvironment context, the effects previously observed in vivo, such as, tumorigenesis attenuation by the alkaloids caffeine and trigoneline, as well as the polyphenol chlorogenic acid individually or in its combinations. Thus, learning novel methods such as tumor stem cells-derived spheroids might accurately be a throughput-screening platform for the understanding of our in vivo ongoing findings.