Genetic, endocrine and transgenerational effects of thermal stress on germinative cells in Rainbow Trout (Oncorhynchus mykiss)

• Grant number: 19/02950-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2019
• End date: January 31, 2020
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Claudio de Oliveira
• Grantee: Arno Juliano Butzge
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

In fish, chronic exposure to high temperatures can affect the proliferation, differentiation or even survival of germ cells, compromising fertility and the reproductive capacity of the species. There is also evidence of epigenetic changes in the DNA of germ cells that may eventually be transmitted to the next generations, determining the survival and adaptive capacity of animals submitted to constant changing environments. This work aims to elucidate the regulatory (molecular and endocrine) and epigenetic mechanisms involved in the maintenance and differentiation of germ cells in animals subjected to prolonged thermal stress using rainbow trout (Oncorhynchus mykiss) as an experimental model. For this purpose, we will perform in vitro and in vivo experiments in which morphological analysis of gonads, gonadal steroid profiles, transcription analysis (qRT-PCR and ISH) of genes related to the spermatogenic process (proliferation and apoptosis), and analysis of DNA methylation in germ cells will be conducted. In addition, will be perform analysis of reproductive performance of animals subjected to high temperatures and zootechnical performance of the offspring of these animals.The results of this study may have implications to species with commercial interest, such as for more efficient development of strains with tolerance to high temperature. For wild populations, they will be important to evaluate long term impacts of climate change and to assess their adaptive capacity under thermal stress. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, MARCOS A.; MARTINEZ, EMANUEL R. M.; BUTZGE, ARNO J.; DORETTO, LUCAS B.; RICCI, JULIANA M. B.; RODRIGUES, MAIRA S.; VIGOYA, ANGEL A. A.; GOMEZ-GONZALEZ, NURIA E.; STEWART, AMANDA B.; NOBREGA, RAFAEL H.. Molecular characterization and expression analysis of anti-Mullerian hormone in common carp (Cyprinus carpio) adult testes. GENE EXPRESSION PATTERNS, v. 40, JUN 2021. (13/03384-4, 14/25313-4, 12/00423-6, 19/02950-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BUTZGE, Arno Juliano. Genetic, endocrine and transgenerational effects of thermal stress on germinative cells in rainbow trout (Oncorhynchus mykiss). 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.