Determination of metabolic alterations and therapeutic potential of Zika Virus in cancer cells by mass spectrometry and artificial intelligence

• Grant number: 19/05718-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): December 01, 2019
• Effective date (End): October 14, 2021
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Rodrigo Ramos Catharino
• Grantee: Jeany Delafiori
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Zika virus (ZIKV) is a flavivirus transmitted by Aedes Aegypti mosquito, which led to a high incidence of cases in Brazil between 2015 and 2016. Although 80% of the cases are asymptomatic, the later association of the disease with neurological complications such as Guillain-Barré syndrome in adults and microcephaly in neonates of infected pregnant women, led to initiatives in the development of diagnostic methods and vaccines, and studies of viral infection process in human and mosquito cells. In parallel, metabolomic strategies associated with mass spectrometry and artificial intelligence have advanced in the research of new therapeutic and diagnostic targets, with more precise and sensitive methodologies. These tools supported advances in ZIKV research, bringing innovation to the elucidation of viral infection biomarkers, understanding of virus mechanisms of action, diagnostic methods and evidences of ZIKV oncolytic potential. The Zika virus tropism for different cell types, including neural and gonads cells, associated with its ability to induce glioblastoma cell death have suggested the possibility of using ZIKV for the treatment of other cancers, such as prostate and ovary cancers. Using in vitro models, the objective is to investigate the therapeutic potential and the metabolic alterations induced by ZIKV through metabolomic studies using mass spectrometry and artificial intelligence. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ARAUJO, FABIO FERNANDES; FARIAS, DAVID DE PAULO; NERI-NUMA, IRAMAIA ANGELICA; DIAS-AUDIBERT, FLAVIA LUISA; DELAFIORI, JEANY; DE SOUZA, FLORISVALDO GAMA; CATHARINO, RODRIGO RAMOS; DO SACRAMENTO, CELIO KERSUL; PASTORE, GLAUCIA MARIA. Gastrointestinal bioaccessibility and bioactivity of phenolic compounds from araca-boi fruit. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 135, JAN 2021. (20/00225-6, 19/05718-3)

DELAFIORI, JEANY; NAVARRO, LUIZ CLAUDIO; SICILIANO, RINALDO FOCACCIA; DE MELO, GISELY CARDOSO; BRANDT BUSANELLO, ESTELA NATACHA; NICOLAU, JOSE CARLOS; SALES, GEOVANA MANZAN; DE OLIVEIRA, ARTHUR NOIN; ALMEIDA VAL, FERNANDO FONSECA; DE OLIVEIRA, DIOGO NOIN; et al. Covid-19 Automated Diagnosis and Risk Assessment through Metabolomics and Machine Learning. Analytical Chemistry, v. 93, n. 4, p. 2471-2479, FEB 2 2021. (20/04705-2, 20/05369-6, 18/10052-1, 19/05718-3, 17/12646-3)

DE ARAUJO, FABIO FERNANDES; FARIAS, DAVID DE PAULO; NERI-NUMA, IRAMAIA ANGELICA; DIAS-AUDIBERT, FLAVIA LUISA; DELAFIORI, JEANY; DE SOUZA, FLORISVALDO GAMA; CATHARINO, RODRIGO RAMOS; DO SACRAMENTO, CELIO KERSUL; PASTORE, GLAUCIA MARIA. Chemical characterization of Eugenia stipitata: A native fruit from the Amazon rich in nutrients and source of bioactive compounds. Food Research International, v. 139, JAN 2021. (20/00225-6, 15/50333-1, 19/05718-3)

FREIRE, LUISA; BRAGA, PATRICIA A. C.; FURTADO, MARIANNA M.; DELAFIORI, JEANY; DIAS-AUDIBERT, FLAVIA L.; PEREIRA, GIULIANO E.; REYES, FELIX G.; CATHARINO, RODRIGO R.; SANT'ANA, ANDERSON S.. From grape to wine: Fate of ochratoxin A during red, rose, and white winemaking process and the presence of ochratoxin derivatives in the final products. FOOD CONTROL, v. 113, JUL 2020. (16/21041-5, 19/05718-3)

LIMA, ESTELA DE OLIVEIRA; NAVARRO, LUIZ CLAUDIO; MORISHITA, KAREN NODA; KAMIKAWA, CAMILA MIKA; MARTINS RODRIGUES, RAFAEL GUSTAVO; DABAJA, MOHAMED ZIAD; DE OLIVEIRA, DIOGO NOIN; DELAHORI, JEANY; DIAS-AUDIBERT, FLAVIA LUISA; RIBEIRO, MARTA DA SILVA; et al. Metabolomics and Machine Learning Approaches Combined in Pursuit for More Accurate Paracoccidioidomycosis Diagnoses. MSYSTEMS, v. 5, n. 3, MAY-JUN 2020. (19/05718-3)

DE SOUZA, FLORISVALDO GAMA; NATHIA-NEVES, GRAZIELLE; DE ARAUJO, FABIO FERNANDES; DIAS AUDIBERT, FLAVIA LUISA; DELAFIORI, JEANY; NERI-NUMA, IRAMAIA ANGELICA; CATHARINO, RODRIGO RAMOS; DE ALENCAR, SEVERINO MATIAS; DE ALMEIDA MEIRELES, MARIA ANGELA; PASTORE, GLAUCIA MARIA. valuation of antioxidant capacity, fatty acid profile, and bioactive compounds from buritirana (Mauritiella armata Mart.) oil: A little-explored native Brazilian frui. Food Research International, v. 142, APR 2021. (15/50333-1, 19/05718-3)

FARIAS, DAVID DE PAULO; DE ARAUJO, FABIO FERNANDES; NERI-NUMA, IRAMAIA ANGELICA; DIAS-AUDIBERT, FLAVIA LUISA; DELAFIORI, JEANY; CATHARINO, RODRIGO RAMOS; PASTORE, GLAUCIA MARIA. Effect of in vitro digestion on the bioaccessibility and bioactivity of phenolic compounds in fractions of Eugenia pyriformis fruit. Food Research International, v. 150, n. A, DEC 2021. (20/00225-6, 19/05718-3, 20/15163-6, 15/50333-1)

OLIVEIRA POVOA, VALQUIRIA MARIANE; DELAFIORI, JEANY; DIAS-AUDIBERT, FLAVIA LUISA; DE OLIVEIRA, ARTHUR NOIN; PASCOAL LOPES, ANA BEATRIZ; DE PAULA, ERICH VINICIUS; BARBOSA PAGNANO, KATIA BORGIA; CATHARINO, RODRIGO RAMOS. Metabolic shift of chronic myeloid leukemia patients under imatinib-pioglitazone regimen and discontinuation. MEDICAL ONCOLOGY, v. 38, n. 9, SEP 2021. (19/05718-3)

DE ARAUJO, FABIO FERNANDES; FARIAS, DAVID DE PAULO; NERI-NUMA, IRAMAIA ANGELICA; DIAS-AUDIBERT, FLAVIA LUISA; DELAFIORI, JEANY; DE SOUZA, FLORISVALDO GAMA; CATHARINO, RODRIGO RAMOS; DO SACRAMENTO, CELIO KERSUL; PASTORE, GLAUCIA MARIA. Influence of high-intensity ultrasound on color, chemical composition and antioxidant properties of araca-boi pulp. Food Chemistry, v. 338, FEB 15 2021. (20/00225-6, 15/50333-1, 19/05718-3)

FARIAS, DAVID DE PAULO; DE ARAUJO, FABIO FERNANDES; NERI-NUMA, IRAMAIA ANGELICA; DIAS-AUDIBERT, FLAVIA LUISA; DELAFIORI, JEANY; CATHARINO, RODRIGO RAMOS; PASTORE, GLAUCIA MARIA. Distribution of nutrients and functional potential in fractions of Eugenia pyriformis: An underutilized native Brazilian fruit. Food Research International, v. 137, NOV 2020. (15/50333-1, 19/05718-3)

DIAS-AUDIBERT, FLAVIA LUISA; NAVARRO, LUIZ CLAUDIO; DE OLIVEIRA, DIOGO NOIN; DELAFIORI, JEANY; MELO, CARLOS FERNANDO ODIR RODRIGUES; GUERREIRO, TATIANE MELINA; ROSA, FLAVIA TRONCON; PETENUCI, DIEGO LIMA; WATANABE, MARIA ANGELICA EHARA; VELLOSO, LICIO AUGUSTO; et al. Combining Machine Learning and Metabolomics to Identify Weight Gain Biomarkers. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 8, JAN 24 2020. (19/05718-3)