Effects of marine pharmaceuticals in ZFL cell line

• Grant number: 19/19939-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2019
• End date: August 31, 2022
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Agreement: Fundação para a Ciência e a Tecnologia (FCT)
• Principal Investigator: Leticia Veras Costa Lotufo
• Grantee: Ives Charlie da Silva
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/07098-0 - Effects of marine pharmaceuticals in zebrafish and ZFL cell line - emphasis, AP.R

Abstract

Antineoplastics is one of the least studied groups concerning environmental impact and represent specific risks to aquatic species due to their mode of action. The questions addressed in the EMPHASIS project are: 1) Do they cause eco-, geno- and cytotoxicity to ZF? 2) What is their bioconcentration potential to ZF? 3) How do they affect the microbiota in ZF? and 4) How antineoplastic exposure alter the metabolic pathway in ZF? To address those questions, the project aims at evaluating the effects of clinic antineoplastics and novel compounds with anticancer properties using in vivo and in vitro models, the potential of cell lines to serve as alternative eco-toxicological models by validating them with the use of in vivo models and to evaluate and validate the ecotoxicological effects of new potential compounds when compared to clinic ones. The Adverse Outcome Pathway approach, will provide robust information linking different organizational levels for use in regulatory risk assessment. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MALAFAIA, GUILHERME; AHMED, MOHAMED AHMED IBRAHIM; DE SOUZA, SINDOVAL SILVA; ESTRELA REZENDE, FERNANDA NEVES; FREITAS, ITALO NASCIMENTO; DA LUZ, THIARLEN MARINHO; DA SILVA, ABNER MARCELINO; CHARLIE-SILVA, IVES; BEZERRA BRAZ, HELYSON LUCAS; BEZERRA JORGE, ROBERTA JEANE; et al. Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata. AQUATIC TOXICOLOGY, v. 245, p. 12-pg., 2022-04-01. (19/19939-1, 20/05761-3)

BELO, MARCO A. A.; OLIVEIRA, MELQUE F.; OLIVEIRA, SUSANA L.; ARACATI, MAYUMI F.; RODRIGUES, LETICIA F.; COSTA, CAMILA C.; CONDE, GABRIEL; GOMES, JULIANA M. M.; PRATA, MARIANA N. L.; BARRA, AYSLAN; et al. Zebrafish as a model to study inflammation: A tool for drug discovery. BIOMEDICINE & PHARMACOTHERAPY, v. 144, DEC 2021. (13/25971-9, 19/19939-1, 18/07098-0)

MENDONCA-GOMES, JULIANA MOREIRA; CHARLIE-SILVA, IVES; GUIMARAES, ABRAAO TIAGO BATISTA; ESTRELA, FERNANDA NEVES; CALMON, MARILIA FREITAS; MICELI, RAFAEL NAVA; SANCHES, PAULO R. S.; BITTAR, CINTIA; RAHAL, PAULA; CILLI, EDUARDO M.; et al. Shedding light on toxicity of SARS-CoV-2 peptides in aquatic biota: A study involving neotropical mosquito larvae (Diptera: Culicidae). Environmental Pollution, v. 289, NOV 15 2021. (19/19939-1, 20/05761-3)

CHARLIE-SILVA, IVES; FEITOSA, NATALIA MARTINS; MENDONCA GOMES, JULIANA MOREIRA; DE MELO HOYOS, DANIELA CHEMIM; MATTIOLI, CRISTIANO CAMPOS; ETO, SILAS FERNANDES; FERNANDES, DAYANNE CARLA; DE ANDRADE BELO, MARCO ANTONIO; SILVA, JULIANA DE OLIVEIRA; BRANCO DE BARROS, ANDRE LUIS; et al. Potential of mucoadhesive nanocapsules in drug release and toxicology in zebrafish. PLoS One, v. 15, n. 9, SEP 24 2020. (19/19939-1, 13/25971-9, 18/07098-0)

VENTURA FERNANDES, BIANCA H.; FEITOSA, NATALIA MARTINS; BARBOSA, ANA PAULA; BOMFIM, CAMILA GASQUE; GARNIQUE, ANALI M. B.; ROSA, IVANA F.; RODRIGUES, MAIRA S.; DORETTO, LUCAS B.; COSTA, DANIEL F.; CAMARGO-DOS-SANTOS, BRUNO; et al. oxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health. Science of The Total Environment, v. 813, MAR 20 2022. (19/00195-2, 18/07098-0, 20/05761-3, 17/17303-7, 20/04680-0, 14/04294-1, 19/21739-0, 19/19939-1, 19/18356-2, 19/14285-3, 19/12234-2)

ROSA, IVANA F.; PECANHA, ANA P. B.; CARVALHO, TABATA R. B.; ALEXANDRE, LEONARDO S.; FERREIRA, VINICIUS G.; DORETTO, LUCAS B.; SOUZA, BEATRIZ M.; NAKAJIMA, RAFAEL T.; DA SILVA, PATRICK; BARBOSA, ANA P.; et al. Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 7, p. 22-pg., 2023-04-01. (20/05761-3, 19/21739-0, 14/04294-1, 19/19939-1, 20/15237-0, 18/07098-0, 21/06742-5)

CHARLIE-SILVA, IVES; FEITOSA, NATALIA M.; PONTES, LETICIA G.; FERNANDES, BIANCA H.; NOBREGA, RAFAEL H.; GOMES, JULIANA M. M.; PRATA, MARIANA N. L.; FERRARIS, FAUSTO K.; MELO, DANIELA C.; CONDE, GABRIEL; et al. Plasma proteome responses in zebrafish following lambda-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts. FRONTIERS IN IMMUNOLOGY, v. 13, p. 21-pg., 2022-09-29. (13/25971-9, 19/19939-1)

CARNEIRO, DIANA; DAMASCENO, EVILA PINHEIRO; FERREIRA, VIOLETA; CHARLIE-SILVA, IVES; TEDIM, JOAO; MAIA, FREDERICO; LOUREIRO, SUSANA; MARTINS, ROBERTO; PAVLAKI, MARIA D.. Zn-Al layered double hydroxides induce embryo malformations and impair locomotion behavior in Danio rerio. NANOIMPACT, v. 30, p. 12-pg., 2023-02-27. (19/19939-1, 18/07098-0)

CASTRO, ANA C. H.; BEZERRA, ITALO R. S.; PASCON, ALINE M.; DA SILVA, GABRIELA H.; PHILOT, ERIC A.; DE OLIVEIRA, VIVIAN L.; MANCINI, RODRIGO S. N.; SCHLEDER, GABRIEL R.; CASTRO, CARLOS E.; DE CARVALHO, LUCIANI R. S.; et al. Modular Label-Free Electrochemical Biosensor Loading Nature-Inspired Peptide toward the Widespread Use of COVID-19 Antibody Tests. ACS NANO, v. 16, n. 9, p. 15-pg., 2022-09-27. (17/02317-2, 19/19939-1, 14/50867-3, 20/05761-3)