Efficacy of drugs associated with nanotechnology for control of cariogenic biofilms

• Grant number: 19/22316-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): January 06, 2020
• Effective date (End): January 05, 2021
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Marlise Inêz Klein Furlan
• Grantee: Guilherme Roncari Rocha
• Supervisor: Danielle Benoit
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Research place: University of Rochester (UR), United States
• Associated to the scholarship: 18/01429-4 - Efficacy of drugs associated with nanotechnology for control of cariogenic biofilms, BP.DR

Abstract

The development of effective therapies for oral biofilm control is challenging. Topical agents used to treat and prevent oral diseases are not kept in the mouth for sufficient time; consequently, they cannot exert their maximum therapeutic potential. Therefore, nanoparticles carrier (NPC) for drug delivery were developed that bind to the surface of hydroxyapatite (the main mineral of teeth), salivary pellicle and exopolysaccharides (main components of cariogenic biofilm' extracellular matrix). These NPCs have responsive elements to pH changes controlling the release of a drug into acidic niches found in pathogenic biofilms. For example, tt-farnesol can be successfully loaded and released by NPCs. The mixed-species biofilm of S. mutans and Candida albicans is extremely pathogenic, with rapid structural development and increased production of matrix rich in exopolysaccharides and of acids via the metabolism of dietary sugars. Hence, the objective is to investigate whether the association with other drugs would help tt-farnesol loaded into NPCs to better control cariogenic biofilm. Thus, the NPCs will be modified (chemistry and structure) to carry other drugs (i.e., myricetin and compound 1771) to perform topical treatments of mixed pathogenic biofilms. The efficacy of the treatments will be demonstrated by microbiological tests, biochemical assays to characterize the matrix, and analysis of biofilm's 3D spatial organization. Statistical tests will be applied according to distribution to interpret the results, adopting a 5% significance level. It is expected to understand how biofilms react to the proposed treatments. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RONCARI ROCHA, GUILHERME; SIMS, JR., KENNETH R.; XIAO, BAIXUE; KLEIN, MARLISE I.; BENOIT, DANIELLE S. W.. Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro. JOURNAL OF ORAL MICROBIOLOGY, v. 14, n. 1, JAN 1 2022. (19/22316-6, 18/01429-4)