Functional evaluation of NANOG gene in segregation of cell lineages in bovine embryos

• Grant number: 19/22649-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2019
• End date: March 31, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcelo Demarchi Goissis
• Grantee: Letícia Escobar Carreiro
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 17/09576-3 - Studies on cell differentiation mechanisms during early bovine embryo development, AP.JP

Abstract

The second event of mammalian cell differentiation is the separation between epiblast (EPI) and hypoblast or primitive endoderm (PE). This occurs shortly after segregation of the internal cell mass (ICM) and the trophectoderm (TE). It is known in mice that NANOG acts oppositely to GATA6 and FGF signaling pathway in EPI/PE segregation. Gene expression analyzes group cell populations so that NANOG and GATA6 expression are mutually exclusive. This project aims to test the hypothesis that NANOG is required for the specification of EPI in bovine embryos. CRISPR/Cas9-mediated gene deletion of NANOG in in vitro produced zygotes is intended. Embryos will then be cultured for blastocyst formation rate, cell distribution, gene and protein expression. It is expected that EPI formation will be inhibited, which will lead to an ICM containing only PE cells. Thus this project would contribute to the understanding of the EPI/PE segregation mechanisms in the bovine species. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARREIRO, LETICIA ESCOBAR; DOS SANTOS, GABRIEL SIQUEIRA; LUEDKE, FELIPE EDUARDO; GOISSIS, MARCELO DEMARCHI. ell differentiation events in pre-implantation mouse and bovine embryo. ANIMAL REPRODUCTION, v. 18, n. 4, 2021. (19/22649-5, 19/21303-8, 19/03014-9, 17/09576-3)

LETÍCIA ESCOBAR CARREIRO; GABRIEL SIQUEIRA DOS SANTOS; FELIPE EDUARDO LUEDKE; MARCELO DEMARCHI GOISSIS. Cell differentiation events in pre-implantation mouse and bovine embryos. Animal Reproduction, v. 18, n. 4, 2022-01-07. (19/21303-8, 19/03014-9, 19/22649-5, 17/09576-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARREIRO, Letícia Escobar. Functional evaluation of NANOG gene in segregation of cell lineages in bovine embryos. 2022. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.