Viral metagenomics of mosquitoes captured in the State of São Paulo

• Grant number: 19/21706-5
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: December 01, 2019
• End date: November 30, 2023
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Agreement: MRC, UKRI ; Newton Fund, with FAPESP as a partner institution in Brazil
• Principal Investigator: Ester Cerdeira Sabino
• Grantee: Vanessa dos Santos Morais
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/14389-0 - Brazil-UK Centre for Arbovirus Discovery, Diagnosis, Genomics and Epidemiology (CADDE), AP.TEM

Abstract

Viruses present in haematophagous insects can be divided into two broad groups: (1) insect viruses that infect vertebrates (arborvirus; arthropod-borne virus) and (2) insect viruses that do not infect them (insect specific viruses). Climatic conditions (hot and humid climate) and the high diversity of hematophagous insect species in Brazil provide more favorable conditions for the emergence and spread of viruses. The introduction of new viruses may also occur in the country, mainly related to trade, tourism or immigration routes and, consequently, the vectors would be able to transmit new viral pathogens, such as the introduction and spread of Zika virus (ZIKV) recently in the Americas. The purpose of this study is to determine the set of viruses (viroma) of mosquitoes captured in the State of São Paulo. To do so, we will compare viroma diversity between two locations that have theoretically geographically isolated mosquito populations. The genomic sequencing used will be Next-Generation Sequencing (NGS) with the Illumina platform. Subsequently, the generated contigs will be pooled with Sequencher software and evaluated for genetic similarity to other existing viruses through BLASTx. Phylogenetic analyzes will be performed, with the new viruses identified, in order to evaluate them for their evolutionary characteristics. Therefore, encouraging research to understand the evolutionary mechanisms, emergence, and spread of mosquito viruses is critical to preventing and controlling the onset or reappearance of viral outbreaks capable of major outbreaks. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ADRIANA LUCHS; LAIS SAMPAIO DE AZEVEDO; ELLEN VIANA DE SOUZA; ROBERTA SALZONE MEDEIROS; YASMIN FRANÇA VIANA PIRES DE SOUZA; DALANE LOUDAL FLORENTINO TEIXEIRA; THIAGO FRANCO DE OLIVEIRA CARNEIRO; GABRIELA MARIA FERNANDES DE ALENCAR; FERNANDA LÚCIA DE SOUSA LEITE MORAIS; DIANA DE FÁTIMA ALVES PINTO; et al. Coxsackievirus A6 strains causing an outbreak of hand-foot-and-mouth disease in Northeastern Brazil in 2018. Revista do Instituto de Medicina Tropical de São Paulo, v. 64, 2022-02-21. (20/02469-0, 17/00021-9, 19/21706-5, 20/14786-0, 20/11182-6)

DE AZEVEDO, LAIS SAMPAIO; FRANCA, YASMIN; VIANA, ELLEN; MEDEIROS, ROBERTA SALZONE; MORILLO, SIMONE GUADAGNUCCI; GUIDUCCI, RAQUEL; RIBEIRO, CIBELE DANIEL; VIEIRA, HELOISA ROSA; BARRIO-NUEVO, KAROLINA MORALES; CUNHA, MARIANA SEQUETIN; et al. Lack of molecular evidence of fecal-borne viruses in capybaras from Sao Paulo state, Brazil, 2018-2020: a minor public health issue. Brazilian Journal of Microbiology, v. N/A, p. 9-pg., 2022-11-07. (20/14786-0, 20/02469-0, 15/12944-9, 20/11182-6, 19/21706-5)

RIBEIRO, GEOVANI DE OLIVEIRA; DA COSTA, ANTONIO CHARLYS; GILL, DANIELLE ELISE; D'ATHAIDE RIBEIRO, EDCELHA SOARES; REGO, MARLISSON OCTAVIO DA S.; MONTEIRO, FRED JULIO COSTA; VILLANOVA, FABIOLA; NOGUEIRA, JULIANA SILVA; MAEDA, ADRIANA YURIKA; DE SOUZA, RENATO PEREIRA; et al. Guapiacu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil. SCIENTIFIC REPORTS, v. 11, n. 1, FEB 25 2021. (19/21706-5, 13/21719-3, 16/01735-2, 12/23645-4, 17/00021-9)

RIBEIRO, GEOVANI DE OLIVEIRA; GILL, DANIELLE ELISE; FORO RAMOS, ENDRYA DO SOCORRO; VILLANOVA, FABIOLA; D'ATHAIDE RIBEIRO, EDCELHA SOARES; COSTA MONTEIRO, FRED JULIO; MORAIS, VANESSA S.; REGO, MARLISSON OCTAVIO DA S.; LIMA ARAUJO, EMERSON LUIZ; PANDEY, RAMENDRA PATI; et al. Chikungunya Virus Asian Lineage Infection in the Amazon Region Is Maintained by Asiatic and Caribbean-Introduced Variants. Viruses-Basel, v. 14, n. 7, p. 14-pg., 2022-07-01. (19/21706-5)

MARCELLE SILVA-SALES; ELCIO LEAL; FLAVIO AUGUSTO DE PÁDUA MILAGRES; RAFAEL BRUSTULIN; VANESSA DOS SANTOS MORAIS; ROBERTA MARCATTI; EMERSON LUIZ LIMA ARAÚJO; STEVEN S. WITKIN; XUTAO DENG; ESTER CERDEIRA SABINO; et al. Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016). Revista do Instituto de Medicina Tropical de São Paulo, v. 62, 2020-12-18. (15/12944-9, 17/00021-9, 19/21706-5)

RIBEIRO, GEOVANI DE OLIVEIRA; MORAIS, VANESSA S.; COSTA MONTEIRO, FRED JULIO; D'ATHAIDE RIBEIRO, EDCELHA SOARES; REGO, MARLISSON OCTAVIO DA S.; PICANCO SOUTO, RAIMUNDO NONATO; VILLANOVA, FABIOLA; TAHMASEBI, ROOZBEH; HEFFORD, PHILIP MICHAEL; DENG, XUTAO; et al. Aedes aegyptifrom Amazon Basin Harbor High Diversity of Novel Viral Species. Viruses-Basel, v. 12, n. 8, AUG 2020. (16/01735-2, 19/21706-5, 17/00021-9)

LUCHS, ADRIANA; TARDY, KAELAN; TAHMASEBI, ROOZBEH; MORILLO, SIMONE GUADAGNUCCI; MILAGRES, FLAVIO AUGUSTO DE PADUA; MORAIS, VANESSA DOS SANTOS; BRUSTULIN, RAFAEL; TELES, MARIA DA APARECIDA RODRIGUES; DE AZEVEDO, LAIS SAMPAIO; DE SOUZA, ELLEN VIANA; et al. Human astrovirus types 1, 4 and 5 circulating among children with acute gastroenteritis in a rural Brazilian state, 2010-2016. ARCHIVES OF VIROLOGY, v. 166, n. 11, AUG 2021. (19/21706-5, 15/12944-9, 17/00021-9, 20/11182-6, 20/02469-0, 16/01735-2)

VILLANOVA, FABIOLA; MARCATTI, ROBERTA; BERTANHE, MAYARA; MORAIS, VANESSA DOS SANTOS; DE PADUA MILAGRES, FLAVIO AUGUSTO; BRUSTULIN, RAFAEL; LIMA ARAUJO, EMERSON LUIZ; TAHMASEBI, ROOZBEH; WITKIN, STEVEN S.; DENG, XUTAO; et al. New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples. MICROORGANISMS, v. 9, n. 7, JUL 2021. (17/00021-9, 19/21706-5)

TINKER, RORY J.; DA COSTA, ANTONIO CHARLYS; TAHMASEBI, ROOZBEH; MILAGRES, FLAVIO AUGUSTO DE PADUA; DOS SANTOS MORAIS, VANESSA; PANDEY, RAMENDRA PATI; JOSE-ABREGO, ALEXIS; BRUSTULIN, RAFAEL; RODRIGUES TELES, MARIA DA APARECIDA; CUNHA, MARIANA SEQUETIN; et al. Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil. ARCHIVES OF VIROLOGY, v. 166, n. 3, p. 905-913, MAR 2021. (17/00021-9, 15/12944-9, 16/01735-2, 19/21706-5)

TAHMASEBI, ROOZBEH; LUCHS, ADRIANA; TARDY, KAELAN; HEFFORD, PHILIP MICHAEL; TINKER, RORY J.; EILAMI, OWRANG; DE PADUA MILAGRES, FLAVIO AUGUSTO; BRUSTULIN, RAFAEL; RODRIGUES TELES, MARIA DA APARECIDA; MORAIS, VANESSA DOS SANTOS; et al. Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics. JOURNAL OF GENERAL VIROLOGY, v. 101, n. 12, p. 1280-1288, 2020. (19/21706-5, 17/00021-9)

RIBEIRO, GEOVANI DE OLIVEIRA; GILL, DANIELLE ELISE; ATHAIDE RIBEIRO, EDCELHA SOARES D.; COSTA MONTEIRO, FRED JULIO; MORAIS, VANESSA S.; MARCATTI, ROBERTA; REGO, MARLISSON OCTAVIO DA S.; ARAUJO, EMERSON LUIZ LIMA; WITKIN, STEVEN S.; VILLANOVA, FABIOLA; et al. Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon. Viruses-Basel, v. 13, n. 4, APR 2021. (17/00021-9, 19/21706-5)

FORO RAMOS, ENDRYA DO SOCORRO; ROSA, ULISSES ALVES; RIBEIRO, GEOVANI DE OLIVEIRA; VILLANOVA, FABIOLA; DE PADUA MILAGRES, FLAVIO AUGUSTO; BRUSTULIN, RAFAEL; MORAIS, VANESSA DOS SANTOS; BERTANHE, MAYARA; MARCATTI, ROBERTA; ARAUJO, EMERSON LUIZ LIMA; et al. High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis. Viruses-Basel, v. 13, n. 4, APR 2021. (19/21706-5, 17/00021-9, 15/12944-9)