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The role of the glycoproteome and phosphoproteome during neuronal differentiation in schizophrenia

Grant number: 19/05155-9
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Start date: January 01, 2020
End date: December 31, 2024
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Daniel Martins-de-Souza
Grantee:Victor Corasolla Carregari
Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:17/25588-1 - From the basic understanding to clinical biomarkers to schizophrenia: a neuroproteomics-centered multidisciplinary study, AP.TEM
Associated scholarship(s):23/04767-6 - Exploring protein post-translational modifications in schizophrenia postmortem brains, BE.EP.PD

Abstract

Schizophrenia is a debilitating mental disorder, which affects approximately 1% of the world population, bringing a significant social and economical burden. Disease symptoms generally manifest in young adults, when the neurodevelopment is at its end. Although many studies have been conducted to understand the origin of schizophrenia, little is known about the molecular mechanisms involved in the disease development. Schizophrenia is the result of genetic dysfunctions since the beginning of neurogenesis and neural development process, which are responsible for the observed symptoms. It is known that Post-Translational-Modification (PTMs) such as Glycosylation and Phosphorylation play a key role in brain development. PTMs dysregulation may lead to defects in the differentiation and migration process of the neural cells. The aim of this project is to perform a glycoproteomic and phosphoproteomics analysis of the total cell extract and membrane proteins of fibroblast-derived induced-pluripotent stem cells from schizophrenia patients and mentally healthy controls during the neural maturation, in order to identify possible PTM changes associated to cell development and communication. After the identification of probable targets, functional assays are going to be performed to validate and have a better comprehension of the role of these PTMs in schizophrenia. This work can contribute to the identification and understanding of the biochemistry and molecular process associated to schizophrenia. (AU)

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Scientific publications (7)
(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)
SILVA-COSTA, LICIA C.; SMITH, BRADLEY J.; CARREGARI, VICTOR CORASOLLA; SOUZA, GUSTAVO H. M. F.; VIEIRA, ERICA M.; MENDES-SILVA, ANA PAULA; ALMEIDA, VALERIA DE; CARVALHO, BENILTON S.; DINIZ, BRENO S.; MARTINS-DE-SOUZA, DANIEL. Plasma proteomic signature of major depressive episode in the elderly. JOURNAL OF PROTEOMICS, v. 269, p. 9-pg., . (14/10068-4, 19/05155-9, 17/18242-1, 20/09165-6, 16/07948-8, 19/05747-3, 17/25588-1, 19/00098-7, 18/03422-7)
SACCON, TATIANA DANDOLINI; MOUSOVICH-NETO, FELIPPE; LUDWIG, RAISSA GUIMARAES; CARREGARI, VICTOR CORASOLLA; DOS ANJOS SOUZA, ANA BEATRIZ; DOS PASSOS, AMANDA STEPHANE CRUZ; MARTINI, MATHEUS CAVALHEIRO; BARBOSA, PRISCILLA PASCHOAL; DE SOUZA, GABRIELA FABIANO; MURARO, STEFANIE PRIMON; et al. SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner. NATURE COMMUNICATIONS, v. 13, n. 1, p. 15-pg., . (20/08716-9, 20/04558-0, 19/05155-9, 21/10373-5, 13/07607-8, 20/04919-2, 20/04746-0, 17/01184-9, 17/23920-9, 16/24163-4, 16/00194-8, 18/21635-8, 19/00098-7, 20/05040-4, 17/08264-8, 20/04579-7, 19/26119-0, 19/04726-2, 20/04583-4, 20/15959-5)
BRUNETTI, NATALIA S.; DAVANZO, GUSTAVO G.; DE MORAES, DIOGO; FERRARI, ALLAN J. R.; SOUZA, GABRIELA F.; MURARO, STEFANIE PRIMON; KNITTEL, THIAGO L.; BOLDRINI, VINICIUS O.; MONTEIRO, LAUAR B.; VIRGILIO-DA-SILVA, JOO VICTOR; et al. SARS-CoV-2 uses CD4 to infect T helper lymphocytes. eLIFE, v. 12, p. 26-pg., . (20/04558-0, 15/15626-8, 19/06459-1, 19/04726-2, 19/16116-4, 19/05155-9, 19/13552-8, 16/18031-8, 19/00098-7, 20/04583-4, 16/00194-8, 21/08354-2, 19/17007-4, 19/22398-2, 17/01184-9, 19/14465-1, 20/04579-7, 13/08293-7, 18/14933-2, 17/23920-9, 19/06372-3, 16/24163-4, 16/23328-0, 20/04919-2, 20/04746-0)
BRANDAO-TELES, CAROLINE; CARREGARI, VICTOR CORASOLLA; REIS-DE-OLIVEIRA, GUILHERME; SMITH, BRADLEY J.; CHAVES, YANE; SOUSA SANTOS, ALINE VALERIA; PINHEIRO, ERICK MARTINS DE CARVALHO; OLIVEIRA, CAIO C.; VIEIRA, ANDRE SCHWAMBACH; CRUNFLI, FERNANDA; et al. Impacts of hnRNP A1 Splicing Inhibition on the Brain Remyelination Proteome. Journal of Neurochemistry, v. 169, n. 1, p. 16-pg., . (19/22398-2, 17/25588-1, 18/01669-5, 23/08885-3, 18/01410-1, 19/05155-9, 23/11514-7)
DE SOUZA, WILLIAM M.; FUMAGALLI, MARCILIO J.; DE LIMA, SHIRLENE T. S.; PARISE, PIERINA L.; CARVALHO, DEYSE C. M.; HERNANDEZ, CRISTIAN; DE JESUS, RONALDO; DELAFIORI, JEANY; CANDIDO, DARLAN S.; CARREGARI, VICTOR C.; et al. Pathophysiology of chikungunya virus infection associated with fatal outcomes. CELL HOST & MICROBE, v. 32, n. 4, p. 26-pg., . (19/24251-9, 17/26908-0, 22/04420-3, 18/14389-0, 19/27333-6, 19/05155-9, 18/17176-8, 17/25588-1, 19/00098-7, 13/08293-7, 19/05718-3)
ANTUNES, ANDRE S. L. M.; DE ALMEIDA, VALERIA; CRUNFLI, FERNANDA; CARREGARI, VICTOR C.; MARTINS-DE-SOUZA, DANIEL; GUEST, PC. Proteomics for Target Identification in Psychiatric and Neurodegenerative Disorders. REVIEWS ON NEW DRUG TARGETS IN AGE-RELATED DISORDERS, PT II, v. 1286, p. 14-pg., . (19/22398-2, 17/25588-1, 17/18242-1, 19/05155-9, 18/03673-0)
REIS-DE-OLIVEIRA, GUILHERME; CARREGARI, VICTOR CORASOLLA; DE SOUSA, GABRIEL RODRIGUES DOS REIS; MARTINS-DE-SOUZA, DANIEL. OmicScope unravels systems-level insights from quantitative proteomics data. NATURE COMMUNICATIONS, v. 15, n. 1, p. 12-pg., . (17/25588-1, 23/04767-6, 18/01410-1, 19/05155-9)