The role of the glycoproteome and phosphoproteome during neuronal differentiation in schizophrenia

• Grant number: 19/05155-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date until: January 01, 2020
• End date until: December 31, 2024
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Daniel Martins-de-Souza
• Grantee: Victor Corasolla Carregari
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 17/25588-1 - From the basic understanding to clinical biomarkers to schizophrenia: a neuroproteomics-centered multidisciplinary study, AP.TEM
• Associated scholarship(s): 23/04767-6 - Exploring protein post-translational modifications in schizophrenia postmortem brains, BE.EP.PD

Abstract

Schizophrenia is a debilitating mental disorder, which affects approximately 1% of the world population, bringing a significant social and economical burden. Disease symptoms generally manifest in young adults, when the neurodevelopment is at its end. Although many studies have been conducted to understand the origin of schizophrenia, little is known about the molecular mechanisms involved in the disease development. Schizophrenia is the result of genetic dysfunctions since the beginning of neurogenesis and neural development process, which are responsible for the observed symptoms. It is known that Post-Translational-Modification (PTMs) such as Glycosylation and Phosphorylation play a key role in brain development. PTMs dysregulation may lead to defects in the differentiation and migration process of the neural cells. The aim of this project is to perform a glycoproteomic and phosphoproteomics analysis of the total cell extract and membrane proteins of fibroblast-derived induced-pluripotent stem cells from schizophrenia patients and mentally healthy controls during the neural maturation, in order to identify possible PTM changes associated to cell development and communication. After the identification of probable targets, functional assays are going to be performed to validate and have a better comprehension of the role of these PTMs in schizophrenia. This work can contribute to the identification and understanding of the biochemistry and molecular process associated to schizophrenia. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SACCON, TATIANA DANDOLINI; MOUSOVICH-NETO, FELIPPE; LUDWIG, RAISSA GUIMARAES; CARREGARI, VICTOR CORASOLLA; DOS ANJOS SOUZA, ANA BEATRIZ; DOS PASSOS, AMANDA STEPHANE CRUZ; MARTINI, MATHEUS CAVALHEIRO; BARBOSA, PRISCILLA PASCHOAL; DE SOUZA, GABRIELA FABIANO; MURARO, STEFANIE PRIMON; et al. SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner. NATURE COMMUNICATIONS, v. 13, n. 1, p. 15-pg., 2022-09-29. (20/08716-9, 20/04558-0, 19/05155-9, 21/10373-5, 13/07607-8, 20/04919-2, 20/04746-0, 17/01184-9, 17/23920-9, 16/24163-4, 16/00194-8, 18/21635-8, 19/00098-7, 20/05040-4, 17/08264-8, 20/04579-7, 19/26119-0, 19/04726-2, 20/04583-4, 20/15959-5)

SILVA-COSTA, LICIA C.; SMITH, BRADLEY J.; CARREGARI, VICTOR CORASOLLA; SOUZA, GUSTAVO H. M. F.; VIEIRA, ERICA M.; MENDES-SILVA, ANA PAULA; ALMEIDA, VALERIA DE; CARVALHO, BENILTON S.; DINIZ, BRENO S.; MARTINS-DE-SOUZA, DANIEL. Plasma proteomic signature of major depressive episode in the elderly. JOURNAL OF PROTEOMICS, v. 269, p. 9-pg., 2022-10-30. (14/10068-4, 19/05155-9, 17/18242-1, 20/09165-6, 16/07948-8, 19/05747-3, 17/25588-1, 19/00098-7, 18/03422-7)

BRUNETTI, NATALIA S.; DAVANZO, GUSTAVO G.; DE MORAES, DIOGO; FERRARI, ALLAN J. R.; SOUZA, GABRIELA F.; MURARO, STEFANIE PRIMON; KNITTEL, THIAGO L.; BOLDRINI, VINICIUS O.; MONTEIRO, LAUAR B.; VIRGILIO-DA-SILVA, JOO VICTOR; et al. SARS-CoV-2 uses CD4 to infect T helper lymphocytes. eLIFE, v. 12, p. 26-pg., 2023-07-31. (20/04558-0, 15/15626-8, 19/06459-1, 19/04726-2, 19/16116-4, 19/05155-9, 19/13552-8, 16/18031-8, 19/00098-7, 20/04583-4, 16/00194-8, 21/08354-2, 19/17007-4, 19/22398-2, 17/01184-9, 19/14465-1, 20/04579-7, 13/08293-7, 18/14933-2, 17/23920-9, 19/06372-3, 16/24163-4, 16/23328-0, 20/04919-2, 20/04746-0)

ANTUNES, ANDRE S. L. M.; DE ALMEIDA, VALERIA; CRUNFLI, FERNANDA; CARREGARI, VICTOR C.; MARTINS-DE-SOUZA, DANIEL; GUEST, PC. Proteomics for Target Identification in Psychiatric and Neurodegenerative Disorders. REVIEWS ON NEW DRUG TARGETS IN AGE-RELATED DISORDERS, PT II, v. 1286, p. 14-pg., 2021-01-01. (19/22398-2, 17/25588-1, 17/18242-1, 19/05155-9, 18/03673-0)