Interaction between the soil microbiome and plant-parasitic nematodes activity in soybean crop

• Grant number: 19/09460-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2020
• End date: January 31, 2023
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Fernando Dini Andreote
• Grantee: Felipe Martins do Rêgo Barros
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 21/12483-2 - The use of molecular techniques to assess nematode biodiversity in Brazilian agricultural soils, BE.EP.DR

Abstract

Nematodes are small animals belonging to the Nematode phylum, which present high soil diversity and perform important environmental functions. Traditionally, the evaluation of nematodes has been carried out using microscopy methods that depend on a high level of knowledge in taxonomy and require too much time. Aiming to overcome these limitations, molecular approaches can be applied in assessing the composition, diversity and abundance of soil nematode community. In Brazil, R $ 16.2 billion is lost annually due to damage caused by plant-parasitic nematodes in the soybean crop. Beneficial soil microorganisms are able to promote the suppression of pathogens, reducing the incidence of root diseases. Although, little is known about the relationship between soil microbial diversity (and activity) and plant-parasitic nematodes activity. However, the objective of this work is to correlate the diversity and microbial activity of the soil with the soil nematode community, which interfere with the soybean crop. For this, samples of soils cultivated with soybean under different cropping systems and different soil types will be analyzed for the diversity and abundance of bacteria, fungi and nematodes through the techniques of Restriction Fragment Length Polymorphisms (T-RFLP), high-throughput sequencing and real-time PCR (qPCR). In parallel, greenhouse experiments will be conducted to evaluate the effect of levels of soil microbial diversity and activity on the activity of Heterodera glycines, Meloidogyne incognita and Pratylenchus brachyurus in soybean plants. The work may be useful for the development of new plant-parasitic nematodes control strategies, as well as adjusting molecular techniques to the study of the nematode community, making possible the rapid and practical evaluation of this component of soil biodiversity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO REGO BARROS, FELIPE MARTINS; PEDRINHO, ALEXANDRE; MENDES, LUCAS WILLIAM; GOMES FREITAS, CAIO CESAR; ANDREOTE, FERNANDO DINI. Interactions between Soil Bacterial Diversity and Plant-Parasitic Nematodes in Soybean Plants. Applied and Environmental Microbiology, v. 88, n. 17, p. 14-pg., 2022-08-24. (19/09460-0)

BARROS, FELIPE MARTINS DO REGO; PEDRINHO, ALEXANDRE; SANT'ANA, GUSTAVO DA CUNHA; FREITAS, CAIO CESAR GOMES; ROSA, JULIANA MAGRINELLI OSORIO; DE OLIVEIRA, CLAUDIO MARCELO GONSALVES; ROZADA, CAIQUE; MARTO, FELIPE NOGUEIRA DE SA; PASCOALINO, JOAO AUGUSTO LOPES; DA SILVA, LUIZ ANTONIO; et al. Plant-parasitic nematode community and enzyme activities in soils under no-till soybean crops in Brazil. RHIZOSPHERE, v. 27, p. 8-pg., 2023-09-01. (19/09460-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BARROS, Felipe Martins do Rêgo. Interaction between soil microbiome and nematode activity in soybean crop. 2023. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.