Antimicrobial activity of proteins secreted by Xanthomonas citri against microorganisms of medical interest

• Grant number: 19/21739-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2020
• End date: December 31, 2021
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Cristiane Rodrigues Guzzo Carvalho
• Grantee: Camila Gasque Bomfim
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Xanthomonas citri (XAC) is a phytopathogen that infects citrus trees, causing citrus canker. This bacterium possesses a secretion system that takes part in the competition for the environment, the Type IV Secretion System (T4SS), which is transmembrane machinery that contains an injection pilus responsible for the delivery of the secreted proteins into other microorganisms. One of these proteins, XAC2609, has been described and shown lysozyme-like activity in vitro assays against bacteria. There are other 12 putative effectors in the system, with domains that range from lipase to lysozyme-like and whose structures and functions have not been described yet. Therefore, in this project we aim to define the best methods for expression and purification of two other proteins secreted by the T4SS, XAC2885 and XAC3634, while also establishing protocols for testing the antibacterial activity of three XAC effectors, XAC2609, XAC2885 and XAC3634, and evaluate their effectiveness against microorganisms of clinical interest.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VENTURA FERNANDES, BIANCA H.; FEITOSA, NATALIA MARTINS; BARBOSA, ANA PAULA; BOMFIM, CAMILA GASQUE; GARNIQUE, ANALI M. B.; ROSA, IVANA F.; RODRIGUES, MAIRA S.; DORETTO, LUCAS B.; COSTA, DANIEL F.; CAMARGO-DOS-SANTOS, BRUNO; et al. oxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health. Science of The Total Environment, v. 813, MAR 20 2022. (19/00195-2, 18/07098-0, 20/05761-3, 17/17303-7, 20/04680-0, 14/04294-1, 19/21739-0, 19/19939-1, 19/18356-2, 19/14285-3, 19/12234-2)

ROSA, IVANA F.; PECANHA, ANA P. B.; CARVALHO, TABATA R. B.; ALEXANDRE, LEONARDO S.; FERREIRA, VINICIUS G.; DORETTO, LUCAS B.; SOUZA, BEATRIZ M.; NAKAJIMA, RAFAEL T.; DA SILVA, PATRICK; BARBOSA, ANA P.; et al. Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 7, p. 22-pg., 2023-04-01. (20/05761-3, 19/21739-0, 14/04294-1, 19/19939-1, 20/15237-0, 18/07098-0, 21/06742-5)