Phytochemical evaluation of apple (Malus domestica) industrial residue from extraction and separation by a two-dimensional system

• Grant number: 19/24537-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): February 01, 2020
• Effective date (End): January 31, 2022
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Maurício Ariel Rostagno
• Grantee: Laise Capelasso da Silva
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2

Abstract

The industrial byproduct of apple has high biological potential due to the presence of bioactive compounds. Advanced extraction techniques should be used to make better use of these compounds. This research project proposes the development of a two-dimensional extraction, separation and analysis system (PLEgrad-SPE x UHPLC) by coupling extraction with gradient pressurized liquids (PLEgrad) with Solid Phase Extraction (SPE) in the first dimension and orthogonally with Ultra-High Pressure Chromatography (UHPLC) in the second dimension. The objective is to develop a system that allows the characterization of the chemical composition of natural raw materials in a fast, efficient and with real-time monitoring, while allowing the production of small quantities of extracts obtained through the fractionation of the present compounds. These fractions should be well characterized chemically and with low contamination between them. The system will be validated with several samples, including soybean (Glycine max), Brazilian ginseng (Pfaffia glomerata), black tea and green tea (Camellia sinensis), mate (Ilex paraguariensis), coffee (Coffea arabica), pomegranate granatum), orange peel (Citrus sinensis), sicilian lemon peel (Citrus x limon) apple peel (Malus domestica), jabuticaba bark (Myrciaria cauliflora), rosemary (Rosmarinus officinalis) and sage (Salvia officinalis). Initially the rapid UHPLC analysis methods will be developed that will be used in the second separation dimension. Several analysis conditions (temperature, column size, flow, volume and injection solvent and mobile phase composition) will be evaluated for an ultra-fast separation method (time between samples < 2.0 min). Subsequently, different extraction conditions will be evaluated by SPE coupled gradient PLE, including temperature, pressure, sample amount, adsorbent type, flow, solvent and duration of the extraction/elution gradient. This proposal consists of a continuation of the Young Researcher project (FAPESP 2013/04304-4), seeking to consolidate this line of research at FCA/UNICAMP and to continue the training of qualified human resources. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIGANO, JULIANE; SANCHES, VITOR L.; MESQUITA, LEONARDO M. DE SOUZA; DE SOUZA, MARIANA C.; DA SILVA, LAISE C.; CHAVES, JAISA O.; FORSTER-CARNEIRO, TANIA; ROSTAGNO, MAURICIO A.. Comprehensive analysis of phenolic compounds from natural products: Integrating sample preparation and analysis. Analytica Chimica Acta, v. 1178, SEP 15 2021. (19/24537-0, 18/14582-5, 19/18772-6, 20/08421-9, 18/17089-8, 13/04304-4, 18/14938-4, 20/15774-5)

DA SILVA, LAISE C.; VIGANO, JULIANE; DE SOUZA MESQUITA, LEONARDO M.; BAIAO DIAS, ARTHUR L.; DE SOUZA, MARIANA C.; SANCHES, VITOR L.; CHAVES, JAISA O.; PIZANI, RODRIGO S.; CONTIERI, LETICIA S.; ROSTAGNO, MAURICIO A.. Recent advances and trends in extraction techniques to recover polyphenols compounds from apple by-products. FOOD CHEMISTRY-X, v. 12, DEC 30 2021. (20/08421-9, 20/03623-2, 19/24537-0, 20/15774-5, 19/18772-6, 19/13496-0, 18/17089-8, 20/04067-6, 18/14582-5)

DA SILVA, LAISE C.; SOUZA, MARIANA C.; SUMERE, BEATRIZ R.; SILVA, LUIZ G. S.; DA CUNHA, DIOGO T.; BARBERO, GERARDO F.; BEZERRA, ROSANGELA M. N.; ROSTAGNO, MAURICIO A.. Simultaneous extraction and separation of bioactive compounds from apple pomace using pressurized liquids coupled on-line with solid-phase extraction. Food Chemistry, v. 318, JUL 15 2020. (18/14582-5, 16/19930-6, 18/17089-8, 19/24537-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Laise Capelasso da. Application of pressurized liquid extraction for purification of bioactive compounds from apple pomace (Malus domestica Borhk): coupling with solid phase extraction and solvent gradient. 2022. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas Limeira, SP.