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Study of plasma proteomic and antioxidant response of broilers supplemented with passion fruit seed oil

Grant number: 19/12179-1
Support type:Scholarships in Brazil - Master
Effective date (Start): January 01, 2020
Effective date (End): February 28, 2021
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal researcher:José Roberto Sartori
Grantee:Fernanda Kaiser de Lima Krenchinski
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Due to the constant seek for new additives to ensure the growing production of chicken meat and the search by consumers for quality meat with functional attributes, the objective of this study is to evaluate the effect of passion fruit seed oil supplementation (OSM) to assess its antioxidant potential by proteomic approach, analysis of enzyme activity of antioxidant system and presence of substances produced during the oxidative stress in broilers. For this purpose, 450 male 1-day-old Cobb® 500 chicks will be used, which will be housed in galvanized wire cages (0.4 x 0.5 x 0.6m) with nipple drinker and trough feeders, in two climatic chambers: thermoneutral and one with cyclic heat stress, with 45 cages each. The design will be completely randomized in a 2 x 5 factorial design, with two temperatures (thermoneutral and cyclic heat stress) and five treatments: control (without OSM inclusion) + four OSM inclusion levels: 0.30; 0.50; 0.70; 0.90%) with nine repetitions of five birds each. The experimental diets will be devoid of antibiotics as a performance enhancer, being used anticoccidial in all treatments. Characterization and determination of the antioxidant capacity of the OSM, determination of the enzyme activity of the antioxidant system in the liver, definition of the antioxidant capacity and lipid peroxidation in the blood and proteomic analysis in the blood plasma of broilers will be performed. The data will be tabulated and analyzed using analysis of variance with the software SAS 9.2 and, when significant (P <0.05), the means levels of inclusion of passion fruit seed oil will be applied (Proc Reg SAS 9.2), and for variables that do not meet the hypothesis of the normality and homogeneity tests, the Kruskal Wallis test will be used, followed by the Chi-square test (P <0.05). (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
KRENCHINSKI, Fernanda Kaiser de Lima. Effects of passion fruit seed oil on the immune and antioxidant systems of broiler. 2021. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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