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Effect of incorporation of cellulose nanocrystals on adhesion and biofilm formation of simple and mixed Candida albicans and methicillin-sensitive Staphylococcus aureus (MSSA) in a denture base resin

Grant number: 19/07491-6
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2020
Effective date (End): April 30, 2021
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal researcher:Ana Carolina Pero
Grantee:Thais de Sousa Santana
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The use of biopolymers from renewable sources as nanometric reinforcing agents can represent an important innovation in dental materials. The aim of this study will be evaluate the simple and mixed biofilm formation of Candida albicans and methicillin-susceptible Staphylococcus aureus (MSSA), on the surface of acrylic resin for denture base, after the incorporation of cellulose nanocrystals (CNC). Cellulose nanocrystals (CNC) isolated from wood pulp will be incorporated into acrylic resin for denture base (Onda-Cryl - OC, activated by microwave energy). Disc-shaped specimens (15 x 3 mm) will be obtained for each condition (groups 0% - control, and 0,75%) Prior to the adhesion and biofilm formation tests, the surface roughness will be measured in a digital rugosimeter Ra (¼m) and the surface free energy will be measured from contact angle measurements, obtained by means of a goniometer connected to a computerized system. The formation of biofilms will be performed from the inoculation of C.albicans and S.aureus in acrylic resin discs incubated at 37ºC for 90 minutes and 48h. After this period, the analysis of cell metabolism test (XTT) will be performed; evaluation of antimicrobial activity by counting colony-forming units (CFU/ml) and analysis by confocal laser microscopy. All analyses will be performed in triplicate on 3 distinct occasions for 90 minutes and 48 hours periods. Data will be tabulated and submitted to adequate statistical tests, according to adherence to normality, at 5% of significance level. (AU)