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Exploring the role of chromatin-associated proteins detected in Trypanosoma cruzi

Grant number: 20/02708-4
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2020
Effective date (End): March 31, 2022
Field of knowledge:Biological Sciences - Biochemistry - Biochemistry of Microorganisms
Principal researcher:Julia Pinheiro Chagas da Cunha
Grantee:Herbert Guimarães de Sousa Silva
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:18/15553-9 - Going deeper into Trypanosoma cruzi chromatin regulation: identifying new players and quizzing its impact on a potential transcription control, AP.JP2

Abstract

To expand the comprehensive of Trypanosoma cruzi chromatin regulation, we proposed to take advantage of cutting-edge methodologies developed to recover proteins associated to a given locus. At this Master's project, we will explore the role of one or two chromatinassociated protein detected at the abovementioned methodology. Ideally, we will select proteins that were enriched at more than one genomic loci. The role of those targets will be explored through CRISPR/Cas9 system for generation of knockouts (KO) parasites and tagged proteins. KO parasites will be evaluated: i. regarding their capacity of proliferation, and differentiation into trypomastigotes metacyclics; and ii. Regarding the effect of gene disruption on chromatin accessibility and transcription rate of the associated locus. Parasites expressing the tagged protein will be used to evaluate its cellular localization and on the discovery of interaction partners by immunoprecipitation (IP) assays. (AU)

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