| Grant number: | 20/01208-8 |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| Start date: | March 01, 2020 |
| End date: | April 30, 2025 |
| Field of knowledge: | Health Sciences - Pharmacy - Pharmaceutical Technology |
| Principal Investigator: | Luciana Biagini Lopes |
| Grantee: | Julia Sapienza Passos |
| Host Institution: | Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated research grant: | 18/13877-1 - Nanocarriers for localized treatment and chemoprevention of breast tumors, AP.JP2 |
| Associated scholarship(s): | 21/12664-7 - Synthesis of thermoresponsive nanostructured lipid carrier core with poly(N-isopropylacrylamide) shell nanoparticle for drug encapsulation and intraductal delivery into the mammary tissue, BE.EP.DD |
Abstract Breast Tumors have a high incidence worldwide, but there are a lack of effective and well-accepted alternatives for localized treatment of noninvasive forms of the disease, such as the Ductal Carcinoma In Situ (DCIS). In this project, we propose the development of polyelectrolyte nanoparticles for intraductal administration of cytotoxic drugs, in order to optimize the drug localization in breast tissue. These complexes will be developed using chitosan and hyaluronic acid, and incorporated into Poloxamer 407, a thermosensitive polymer, in order to obtain in vivo gelling systems, aiming to prolong the residence time of nanoparticles in the mammary ducts. The affinity between composition and drug incorporation will be studied in order to evaluate how composition affects the properties of the system, by characterization by particle size, polydispersion index and zeta potential. A formulation will then be selected (optimized formulation) to evaluate the bioadhesive properties, irritation potential, drug release and time of residence in the breast tissue. The influence of nanoencapsulation on its efficacy and selectivity will be evaluated on 2D tumor cultures and on 3D multicellular spheroids, by studying formulation influence on viability and proliferation of tumor and non-transformed cells. With this study, we aim to contribute to the development of a more effective and safe platform for the treatment of DCIS. (AU) | |
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