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Role of chemokine receptor 5 (CCR5) in apical periodontitis etiopathogenesis

Grant number: 19/13860-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2019
Effective date (End): August 31, 2020
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Andiara de Rossi
Grantee:Fernanda Carolina Jacomini
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Interest in the study of the CC 5 (CCR5) chemokine receptor has increased since it is involved not only in the infection of the organism and migration of leukocytes to sites of inflammation but also in cell signaling, including osteoblasts and osteoclasts, and currently plays a therapeutic role In various human diseases. However, the role of CCR5 in the protection or stimulation of tissue resorption associated with apical periodontitis has not yet been elucidated. The present research project proposes to characterize the involvement of CCR5 in the genesis and progression of apical periodontitis, using animals deficient for this molecule. Slides obtained from the slide bank of the Department of Child Clinic of the Ribeirao Preto School of Dentistry were used, in which experimental induction of periapical lesion was performed in the lower molars of CCR5 -/- (knockout) transgenic mice and wild type mice. who underwent euthanasia at 0, 7, 21 and 42 days. The slides will be evaluated by conventional microscopy to evaluate the presence of inflammatory cells in the presence or absence of CCR5 (neutrophils, macrophages and CD4 and CD8 lymphocytes) and tissue infection. It is expected to characterize the role of CCR5 in apical periodontitis and, in the future, to apply molecular treatments directed to the silencing of this cell signaling pathway, as is currently occurring in several inflammatory diseases, including periodontal disease.