Substitution of bovine fetal serum for cryopreservation of mesenchymal stromal cells from canine adipose tissue

• Grant number: 20/01533-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2020
• Effective date (End): December 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Fernanda da Cruz Landim
• Grantee: Gabriel Corrêa de Camargo
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Mesenchymal stromal cells (MSCs) are multipotent cells isolated from different animal tissues, which are capable of performing therapeutic functions, acting in several human and animal pathologies, when used in cell therapy protocols. In order to optimize their use and enable the creation of cryogenic banks containing MSCs, laboratories around the world use protocols containing cryoprotective substances in association with macromolecules, among which the most used is Bovine Fetal Serum (FBS). The use of FBS can bring several deleterious effects in relation to individuals undergoing cell therapy who will receive material containing serum molecules, such as anaphylactic reactions and transmission of prionic diseases, in addition to this substance bringing risks of contamination during cell culture itself. In this sense, it is pertinent to develop protocols that replace FBS, as an auxiliary macromolecule, in the cryopreservation process of MSCs, with Bovine Albumin, the Knockout " serum substitute and Polyvinyl Alcohol components with the potential to perform the functions of the FBS and replace it in its performance in the cell cryopreservation process.