Systematic and distribution of myxozoans collected in fish from Pardo River, middle Paranapanema River, State of São Paulo

• Grant number: 19/19060-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2020
• End date: April 11, 2023
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
• Principal Investigator: Reinaldo José da Silva
• Grantee: Diego Henrique Mirandola Dias Vieira
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 22/06703-2 - Studies on the life cycle of myxozoans and integrative taxonomy of species in Brazil, BE.EP.PD

Abstract

Parasites of aquatic organisms are of considerable relevance to study because of their impact on Aquaculture systems and fisheries worldwide and its biodiversity not yet known. Myxozoans are parasites that have a complex life cycle, parasitizing a vertebrate host, mainly fish, alternating with an invertebrate host, these annelids. Myxozoans are among the agents responsible for causing fish diseases and can cause major economic losses, with high mortality rates. Myxozoa taxonomy is traditionally done through morphological and molecular studies. Phylogenetic molecular studies for this parasite group make extensive use of ribosomal genes, especially the SSU rDNA, and often these genes are incipient in resolving group relationships. A new approach, such as phylogenomics through the use of Ultra Conserved Elements (UCEs), brings an analysis that is beginning to be used for this group of parasites. The systematics and phylogeography of invertebrates through the study of ultraconserved markers has recently been used to obtain genomic data capable of elucidating some relationships between species of the various families belonging to the Myxozoa. The present work aims to study the systematics, biodiversity and distribution of myxosporideans found in fish from Pardo River. The Pardo River is part of the Corumbataí-Botucatu-Tejupá Environmental Protection Area, created for sustainable management and environmental recovery actions in the region, as well as recharge areas of the Guarani Aquifer, the largest underground freshwater source in the world. For the study will be developed morphological analysis from light and electron microscopy, molecular analysis using traditional ribosomal markers and sequencing of new generation using UCEs. The phylogenetic relationships of myxozoans parasites of fishes from Rio Pardo and the general evolutionary context of this group will be analyzed based on the results obtained through sequencing. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIRANDOLA DIAS VIEIRA, DIEGO HENRIQUE; NARCISO, RODRIGO BRAVIN; DE AZEVEDO, RODNEY KOZLOWISKI; DA SILVA, REINALDO JOSE. Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses. ACTA PARASITOLOGICA, JUL 2021. (19/26831-2, 20/05412-9, 19/19060-0)

NARCISO, RODRIGO BRAVIN; MIRANDOLA DIAS VIEIRA, DIEGO HENRIQUE; DA SILVA, REINALDO JOSE. A new species of Tiddergasilus Marques & Boeger, 2018 (Copepoda: Ergasilidae) from the gills of Astyanax lacustris (Lutken) (Osteichthyes: Characidae) in Brazil. Systematic Parasitology, v. 99, n. 6, p. 11-pg., 2022-07-14. (19/19060-0, 20/05412-9, 19/26831-2)

UNGARI, LETICIA PEREIRA; VIEIRA, DIEGO HENRIQUE MIRANDOLA DIAS; DE ALCANTARA, EDNA PAULINO; EMMERICH, ENZO; SANTOS, ANDRE LUIZ QUAGLIATTO; DA SILVA, REINALDO JOSE; O'DWYER, LUCIA HELENA. Description of a new species of myxobolid parasite, Henneguya pindaibensis n. sp. (Cnidaria: Myxosporea), infecting the gills of Boulengerella cuvieri (Spix and Agassiz, 1829) from Brazil. Parasitology International, v. 83, AUG 2021. (18/00754-9, 18/09623-4, 19/19060-0)

MIRANDOLA DIAS VIEIRA, DIEGO HENRIQUE; UNGARI, LETICIA PEREIRA; DE ALCANTARA, EDNA PAULINO; EMMERICH, ENZO; QUAGLIATTO SANTOS, ANDRE LUIZ; O'DWYER, LUCIA HELENA; DA SILVA, REINALDO JOSE. Description of Cystodiscus elachistocleis sp. nov. (Cnidaria: Myxosporea) parasitizing the gallbladder of Elachistocleis cesarii from Brazil, based on morphological and molecular analyses. EUROPEAN JOURNAL OF TAXONOMY, v. 775, p. 107-118, OCT 21 2021. (19/19060-0, 18/00754-9, 18/09623-4)

DE AZEVEDO, RODNEY KOZLOWISKI; NEGRELLI, DEBORA CAROLINE; DE OLIVEIRA, CRISTIANO PINTO; ABDALLAH, VANESSA DORO; SARMENTO CAMARA, JULIANA PEREIRA; MATOS, EDILSON RODRIGUES; MIRANDOLA DIAS VIEIRA, DIEGO HENRIQUE. Morphological and molecular analysis of Henneguya lagunensis n. sp. (Cnidaria, Myxosporea) parasitizing the gills of Eugerres brasilianus from Brazil. Parasitology International, v. 80, FEB 2021. (19/19060-0)

MIRANDOLA DIAS VIEIRA, DIEGO HENRIQUE; NARCISO, RODRIGO BRAVIN; DA SILVA, REINALDO JOSE. Diversity of myxozoans parasitizing the catfish Rhamdia quelen (Siluriformes: Heptapteridae), in southeastern Brazil, based on morphological and molecular evidence. SCIENTIFIC REPORTS, v. 12, n. 1, p. 10-pg., 2022-10-20. (19/19060-0, 19/26831-2)

UNGARI, L. P.; VIEIRA, D. H. M. D.; SANTOS, A. L. Q.; DA SILVA, R. J.; O'DWYER, L. H.. Myxobolus spp. (Cnidaria: Myxobolidae) no sangue circulante de peixes dos estados de Goiás e Mato Grosso, Brasil: relato de caso. Brazilian Journal of Biology, v. 82, 2022. (18/00754-9, 18/09623-4, 19/19060-0)

VIEIRA, DIEGO HENRIQUE MIRANDOLA DIAS; RANGEL, LUIS FILIPE; TAGLIAVINI, VINICIUS PANCIERA; ABDALLAH, VANESSA DORO; SANTOS, MARIA JOAO; DE AZEVEDO, RODNEY KOZLOWISKI. A new species,Henneguya lacustrisn. sp. (Cnidaria: Myxosporea), infecting the gills ofAstyanax lacustrisfrom Brazil. Parasitology Research, v. 119, n. 12, SEP 2020. (19/07708-5, 14/12862-0, 15/24901-2, 19/19060-0)

OSAKI-PEREIRA, MELISSA MIYUKI; NARCISO, RODRIGO BRAVIN; MIRANDOLA DIAS VIEIRA, DIEGO HENRIQUE; MUELLER, MARIA ISABEL; EBERT, MARIANA BERTHOLDI; DA SILVA, REINALDO JOSE. Molecular phylogeny of two Rhinoxenus species (Monogenea: Dactylogyridae) from the nasal cavities of serrasalmids (Characiformes: Serrasalmidae) from Brazil. Systematic Parasitology, v. 100, n. 5, p. 10-pg., 2023-06-20. (21/12779-9, 17/16546-3, 19/19060-0, 20/05412-9, 19/26831-2)

NARCISO, RODRIGO BRAVIN; MIRANDOLA DIAS VIEIRA, DIEGO HENRIQUE; ALVES DIAS, KARINA GABRIELE; ABDALLAH, VANESSA DORO; DA SILVA, REINALDO JOSE. Redescription of Wallinia brasiliensis (Digenea: Allocreadiidae) from Astyanax lacustris (Osteichthyes: Characidae) in Brazil. BIOLOGIA, v. 77, n. 4, p. 10-pg., 2022-03-10. (20/05412-9, 19/19060-0, 19/26831-2)

DIAS VIEIRA, DIEGO HENRIQUE MIRANDOLA; NARCISO, RODRIGO BRAVIN; DA SILVA, REINALDO JOSE. Morphological and molecular characterization of the cryptic species Myxobolus cataractae n. sp. (Cnidaria: Myxozoa: Myxobolidae) parasitizing Imparfinis mirini (Siluriformes: Heptapteridae). Parasitology International, v. 88, p. 8-pg., 2022-02-16. (20/05412-9, 19/19060-0, 19/26831-2)