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Epigenetic susceptibility to periodontal disease

Grant number: 19/20103-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2020
Effective date (End): November 30, 2022
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Daniela Bazan Palioto Bulle
Grantee:Cristhiam de Jesus Hernandez Martinez
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The purpose of these studies is to analyze the differences in susceptibility to Periodontal Disease (PD) after systemic microbial challenge in strains of mice known to be susceptible (BALB/C) and resistant (C57BL/6). For the first and second objective, 140 mice will be used, divided into 2 groups (n = 70 BALB/c & n = 70 C57BL/6). Each group will be divided into 3 subgroups: Control (C), Sham vehicle with 2% sterile carboxymethylcellulose (Sham V), Porfiromonas gingivalis W83 (GPgW83). The microbial challenge will be carried out weekly for 3 weeks, and after this period, the mice will be kept until completing 42 days of experiments when, then, they will be euthanized. Will be evaluated: 1) alveolar bone microarchitecture in the upper 2nd molar region by means of x-ray computed microtomography (Micro-CT); 2) severity of inflammation present in the interproximal region of the upper second molar through histomorphometric analysis; 3) expression of epigenetic methylation markers; (DNMT3b) and histone acetylation acetyl histone 3 (H3) and acetyl histone 4 (H4) by immunohistochemistry in the intestine and maxilla; 4) methylation profile (methylation array). The data obtained will be submitted to statistical analysis (p <0.05). (AU)

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