Epigenetic susceptibility to periodontal disease

• Grant number: 19/20103-5
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2020
• Effective date (End): November 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal researcher: Daniela Bazan Palioto Bulle
• Grantee: Cristhiam de Jesus Hernandez Martinez
• Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The purpose of these studies is to analyze the differences in susceptibility to Periodontal Disease (PD) after systemic microbial challenge in strains of mice known to be susceptible (BALB/C) and resistant (C57BL/6). For the first and second objective, 140 mice will be used, divided into 2 groups (n = 70 BALB/c & n = 70 C57BL/6). Each group will be divided into 3 subgroups: Control (C), Sham vehicle with 2% sterile carboxymethylcellulose (Sham V), Porfiromonas gingivalis W83 (GPgW83). The microbial challenge will be carried out weekly for 3 weeks, and after this period, the mice will be kept until completing 42 days of experiments when, then, they will be euthanized. Will be evaluated: 1) alveolar bone microarchitecture in the upper 2nd molar region by means of x-ray computed microtomography (Micro-CT); 2) severity of inflammation present in the interproximal region of the upper second molar through histomorphometric analysis; 3) expression of epigenetic methylation markers; (DNMT3b) and histone acetylation acetyl histone 3 (H3) and acetyl histone 4 (H4) by immunohistochemistry in the intestine and maxilla; 4) methylation profile (methylation array). The data obtained will be submitted to statistical analysis (p <0.05). (AU)