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Comparative analysis of the alveolar bone repair process in male mice of lines 129Sv/EV, C57BL/6J and BALB/c

Grant number: 20/03330-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2020
Effective date (End): May 31, 2021
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Mariza Akemi Matsumoto
Grantee:Ana Carolyna Becher Roseno
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The use of small rodents as tools for investigation of events related to human health is already established, especially mice. The inbred variety of these animals allows the selection of the more adequate one for a specific target to be studied; however, in some circumstances this characteristic is not considered, especially when no genetic manipulation is necessary. In this way, this study aimed to characterize alveolar bone repair of three different mice inbred, C57BL/6J, 129/Sv e BALB/c, due to their differences in the capacity of inflammation response. Sixty mice will be used, C57Bl/6, 129/Sv e BALB/c (n=20 for each one), aged between 12 to 16 months, mean weight of 28 grams (C57Bl/6, 129/Sv) and 40 grams (BALB/c), and undergo upper right incisor extraction. After 7 and 30 post-operative days, the animals will be euthanized for the removal of the maxillae containing the healing sockets for descriptive histopathological analysis, histomorphometry considering the number of granulation tissue, newly bone tissue, neutrophils, lymphocytes and plasma cells, from the analysis of the histological slices stained with hematoxylin and eosin (H&E), and immune-inflammatory targets iNOS, and TGF-B using immunohistochemistry technique. The quantification of the structures and cells will be made using ImageJ software. The obtained results will undergo normality distribution tests for the selection of the adequate statistical tests. Statistical assessment will be performed considering significance level of 5% (pd0.05), using Prism5 software (GraphPad, San Diego, CA, USA).