Evaluation of cytotoxic or neuroprotective effects of altered peptides on the central nervous system of zebrafish (Danio rerio) in Parkinson's Disease

• Grant number: 19/17433-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2020
• End date: June 30, 2021
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Leandro Mantovani de Castro
• Grantee: Louise Oliveira Fiametti
• Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil

Abstract

Parkinson's disease is the second most common neurodegenerative disease worldwide, affecting about 7 to 10 million people, characterized by the loss of dopaminergic neurons, leading to uncontrolled voluntary movement causing tremors, postural instability, joint stiffness and difficulty in speech and locomotion, among other symptoms. Its cause remains unknown, which leads to a large number of research. An experimental model that has gained space in the scientific environment is the zebrafish (Danio rerio), a small teleost fish with about 70% of genes homologous to humans. Recently, studies have demonstrated the participation of intracellular peptides in other neurodegenerative diseases. In this context, this study aims to evaluate the altered peptides in the zebrafish brain induced to parkinsonian conditions as to their cytotoxic or neuroprotective action, as well as protein-protein interaction targets and cell signaling pathways.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FIAMETTI, LOUISE OLIVEIRA; CORREA, CLAUDIA NEVES; CASTRO, LEANDRO MANTOVANI DE. Peptide Profile of Zebrafish Brain in a 6-OHDA-Induced Parkinson Model. ZEBRAFISH, v. 18, n. 1, FEB 2021. (19/17433-3, 19/16023-6)

CORREA, CLAUDIA NEVES; FIAMETTI, LOUISE OLIVEIRA; MAZZI ESQUINCA, MARIA EDUARDA; DE CASTRO, LEANDRO MANTOVANI. Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 177, NOV 2021. (21/01286-1, 19/17433-3, 19/16023-6)