Use of photodynamic therapy, beta-tricalcium phosphate and doxycycline in the alveolar repair of rats induced by osteonecrosis: microtomographical and laser confocal microscopy analysis

• Grant number: 19/27609-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2020
• End date: June 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Francisley Ávila Souza
• Grantee: Letícia Gabriella de Souza Rodrigues
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Bisphosphonates are anti-resorptive drugs used in the treatment of skeletal disorders, such as osteoporosis, but their prolonged use may induce osteonecrosis of the jaw (MRONJ), which represents a challenging treatment for dentistry. The aim of this project will be to evaluate the preventive effect of therapies on MRONJ during the alveolar repair process in rats. For this, 360 wistar rats will be used, which will be treated with 04 applications of 0.035 mg / kg of zoledronic acid (ZOE) through the caudal vein with 15 days intervals between applications and subsequently have their right lower molars removed. After extraction, the animals will continue treatment with ZOE in the same protocol until euthanasia. The animals will be divided into seven groups according to the treatment of the dental socket: GS (Sham - ZOE will apply), GC (blood clot only), G² (²-tricalcium phosphate), GD (10% doxycycline), GP (photo-dynamic therapy), GD² (10% doxycycline + ²-tricalcium phosphate), GP² (photo-dynamic therapy + ²-tricalcium phosphate), GPD (photo-dynamic therapy + 10% doxycycline), GPD² (photo-dynamic therapy + 10% doxycycline + ²-tricalcium phosphate). . For in vivo analysis, in the calcified cuts will be used 72 animals, where after 36 days from the date of extraction, the animals will be euthanized for evaluation by 1) application of fluorochromes (calcein and alizarin) followed by confocal laser microscopy analysis. and 2) microtomographic analysis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HADAD, HENRIQUE; KAWAMATA DE JESUS, LAIS; PIQUERA SANTOS, ANA FLAVIA; RINALDI MATHEUS, HENRIQUE; DE SOUZA RODRIGUES, LETICIA GABRIELLA; PAOLO POLI, PIER; MARCANTONIO JUNIOR, ELCIO; POZZI SEMEGHINI GUASTALDI, FERNANDO; MAIORANA, CARLO; MILANEZI DE ALMEIDA, JULIANO; et al. Beta tricalcium phosphate, either alone or in combination with antimicrobial photodynamic therapy or doxycycline, prevents medication-related osteonecrosis of the jaw. SCIENTIFIC REPORTS, v. 12, n. 1, p. 14-pg., 2022-10-03. (19/22657-8, 19/27609-1)