Evolutionary relationships and demographic history in Loricariidae (Siluriformes) species, with emphasis on Harttia genus

• Grant number: 20/02681-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): August 01, 2020
• Status: Discontinued
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Marcelo de Bello Cioffi
• Grantee: Francisco de Menezes Cavalcante Sassi
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated scholarship(s): 22/04261-2 - Integrating cytogenetics and genomics: evolution of sex chromosome systems in Harttia (Siluriformes, Loricariidae) species, BE.EP.DR

Abstract

Geographic barriers and genetic mechanisms are mainly responsible for reproductive isolation in several species. Chromosomal rearrangements, especially involving the sexual pair, are determinants to postzygotic isolation. Fishes known as "cascudos" and "acaris" belong to Loricariidae family and presents a huge karyotypic variation, with diploid numbers varying from 36 to 74 chromosomes per cell, in addition to both simple and multiple sex chromosome systems in several species. Between the members of this family, the genus Harttia is highlighted, inhabiting small streams of Neotropical region, with a special emphasis for Brazil, that encompasses the greatest diversity. Considering all 27 described species, only eight presents cytogenetical data. However, two sex chromosome systems and a variation of 52 to 62 chromosomes are described, which indicates that this groups fits as a good model for evolutionary studies. By that, we aim to perform an analysis on Loricariidae species, with emphasis on Harttia genus, whose results obtained will be compared with chromosomal, genomic and evolutionary divergence time data. For this, classical cytogenetic experiments (Giemsa and c-banding) and molecular (chromosomal painting, comparative genomic hybridization, DNA mapping by FISH), allied to DNA sequencing (DArT-Seq) and bioinformatic analysis to: i) perform an evolutionary cytogenomics analysis in Harttia species and in phylogenetic related groups; ii) investigate the origin of sex chromosome systems in Harttia; iii) analyze the impact of sex chromosome systems in genetic diversity and; iv) trace the demographic history and phylogeography of Harttia. This project presents a continuity on studies of Fish Cytogenetics Lab in Siluriformes members, contributing to a better understand of genetic mechanisms behind the evolutionary process. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROSA DE MORAES, RENATA LUIZA; CAVALCANTE SASSI, FRANCISCO DE MENEZES; CARLOS BERTOLLO, LUIZ ANTONIO; FERREIRA MARINHO, MANOELA MARIA; VIANA, PATRIK FERREIRA; FELDBERG, ELIANA; SALES OLIVEIRA, VANESSA CRISTINA; DEON, GEIZE APARECIDA; AL-RIKABI, AHMED B. H.; LIEHR, THOMAS; et al. Tracking the Evolutionary Trends Among Small-Size Fishes of the Genus Pyrrhulina (Characiforme, Lebiasinidae): New Insights From a Molecular Cytogenetic Perspective. FRONTIERS IN GENETICS, v. 12, OCT 6 2021. (20/11772-8, 20/02681-9, 19/25045-3)

SASSI, FRANCISCO DE M. C.; MOREIRA-FILHO, ORLANDO; DEON, GEIZE A.; SEMBER, ALEXANDR; BERTOLLO, LUIZ A. C.; LIEHR, THOMAS; OLIVEIRA, VANESSA C. S.; VIANA, PATRIK F.; FELDBERG, ELIANA; VICARI, MARCELO R.; et al. Adding New Pieces to the Puzzle of Karyotype Evolution in Harttia (Siluriformes, Loricariidae): Investigation of Amazonian Species. BIOLOGY-BASEL, v. 10, n. 9, SEP 2021. (20/11772-8, 20/02681-9)

SASSI, FRANCISCO DE M. C.; DEON, GEIZE A.; MOREIRA-FILHO, ORLANDO; VICARI, MARCELO R.; BERTOLLO, LUIZ A. C.; LIEHR, THOMAS; DE OLIVEIRA, EZEQUIEL AGUIAR; CIOFFI, MARCELO B.. Multiple Sex Chromosomes and Evolutionary Relationships in Amazonian Catfishes: The Outstanding Model of the Genus Harttia (Siluriformes: Loricariidae). GENES, v. 11, n. 10, OCT 2020. (18/22033-1, 20/02681-9)

DE M. C. SASSI, FRANCISCO; PEREZ, MANOLO F.; OLIVEIRA, VANESSA CRISTINA S.; DEON, GEIZE A.; DE SOUZA, FERNANDO H. S.; FERREIRA, PEDRO H. N.; DE OLIVEIRA, EZEQUIEL A.; HATANAKA, TERUMI; LIEHR, THOMAS; BERTOLLO, LUIZ A. C.; et al. High Genetic Diversity despite Conserved Karyotype Organization in the Giant Trahiras from Genus Hoplias (Characiformes, Erythrinidae). GENES, v. 12, n. 2, FEB 2021. (18/24235-0, 18/22033-1, 20/02681-9)

DEON, GEIZE APARECIDA; GLUGOSKI, LARISSA; VICARI, MARCELO RICARDO; NOGAROTO, VIVIANE; SASSI, FRANCISCO DE MENEZES CAVALCANTE; CIOFFI, MARCELO DE BELLO; LIEHR, THOMAS; BERTOLLO, LUIZ ANTONIO CARLOS; MOREIRA-FILHO, ORLANDO. Highly Rearranged Karyotypes and Multiple Sex Chromosome Systems in Armored Catfishes from the Genus Harttia (Teleostei, Siluriformes). GENES, v. 11, n. 11, NOV 2020. (18/22033-1, 20/02681-9)