Effects of copper oxide nanoparticles using in vitro and in vivo bioassays with lettuce (Lactuca sativa L.)

• Grant number: 19/26337-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2020
• End date: May 31, 2021
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Tatiane Araujo de Jesus
• Grantee: Marcio Yukihiro Kohatsu
• Host Institution: Centro de Engenharia, Modelagem e Ciências Sociais Aplicadas (CECS). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

The increase in the agricultural sector related to the grain harvest and the increase in planted area is mainly due to the applied technologies, such as the use of several products to increase productivity and inhibit plant pathogens. One of the technologies employed is nanoparticles (NPs). Several NPs, such as copper oxide (CuO NPs) are already widely used commercially. However, they can cause several toxic effects, such as: germination inhibition, reduction of root elongation, increased synthesis of metabolites and reactive oxygen species, reduction of chlorophyll and carotenoids. However, phytotoxicity depends on several factors, such as: dose, synthesis, size and composition of NP and seed sensitivity. Thus, in order for NP to actually act in increasing productivity, it is necessary to determine the optimal concentration to be applied. In the present project will be used a biogenic NP (synthesized with green tea extract), aiming, in addition to increasing productivity, reducing phytotoxic effects. In addition, CuSO4 will be used for comparison with NP. Considering this context, the present project aims to evaluate and compare the impact of NP of copper oxide on lettuce (Lactuca sativa) through in vitro (Petri dishes) and in vivo (cultivable soil) bioassays, with n = 6. Will be The elongation and oxidative stress of lettuce seed rootlets were evaluated in in vitro bioassays, as well as lettuces (in vivo bioassays). In addition, the concentrations of macro and microelements present in lettuce and soil will be determined. Photosynthetic pigments (chlorophyll and carotenoids) and primary metabolites (total phenolics and flavonoids) in lettuces will also be evaluated. This study aims to contribute to advances in agronomical technology, using a more sustainable nanomaterial. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KOHATSU, MARCIO YUKIHIRO; PELEGRINO, MILENA TREVISAN; MONTEIRO, LUCILENA REBELO; FREIRE, BRUNA MOREIRA; PEREIRA, RODRIGO MENDES; FINCHEIRA, PAOLA; RUBILAR, OLGA; TORTELLA, GONZALO; BATISTA, BRUNO LEMOS; DE JESUS, TATIANE ARAUJO; et al. Comparison of foliar spray and soil irrigation of biogenic CuO nanoparticles (NPs) on elemental uptake and accumulation in lettuce. Environmental Science and Pollution Research, v. 28, n. 13, p. 16350-16367, APR 2021. (17/05029-8, 19/26337-8, 16/10060-9, 17/20914-8, 18/08194-2)

KOHATSU, MARCIO YUKIHIRO; PELEGRINO, MILENA TREVISAN; MONTEIRO, LUCILENA REBELO; FREIRE, BRUNA MOREIRA; PEREIRA, RODRIGO MENDES; FINCHEIRA, PAOLA; RUBILAR, OLGA; TORTELLA, GONZALO; BATISTA, BRUNO LEMOS; DE JESUS, TATIANE ARAUJO; et al. Comparison of foliar spray and soil irrigation of biogenic CuO nanoparticles (NPs) on elemental uptake and accumulation in lettuce. APL BIOENGINEERING, v. 5, n. 1, p. 16350-16367, MAR 1 2021. (19/26337-8, 16/10060-9, 17/05029-8, 17/20914-8, 18/08194-2)

KOHATSU, MARCIO YUKIHIRO; LANGE, CAMILA NEVES; PELEGRINO, MILENA TREVISAN; PIERETTI, JOANA CLAUDIO; TORTELLA, GONZALO; RUBILAR, OLGA; BATISTA, BRUNO LEMOS; SEABRA, AMEDEA BAROZZI; DE JESUS, TATIANE ARAUJO. Foliar spraying of biogenic CuO nanoparticles protects the defence system and photosynthetic pigments of lettuce (Lactuca sativa). JOURNAL OF CLEANER PRODUCTION, v. 324, NOV 15 2021. (19/26337-8, 17/20914-8, 17/05029-8, 21/00738-6, 18/08194-2, 16/10060-9)