Bioprospection of bacteriocinogenic probiotic bacteria from the intestinal tract of swine (Sus scrofa domesticus):antimicrobial effect against Escherichia coli

• Grant number: 20/03071-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2020
• Effective date (End): March 31, 2022
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Ricardo Pinheiro de Souza Oliveira
• Grantee: Sara Mariano Franco
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems, AP.TEM

Abstract

Swine are important constituents of the human diet, occupying the second place in the ranking of the most consumed in the world, however, the technology of intensive animal production allows the emergence of infectious diseases. The use of probiotic microorganisms and their biomolecules, such as bacteriocins, in the prevention and treatment of bacterial infections in animals intended for human consumption, such as swine, has been considered as an efficient alternative to the use of antimicrobials. In this context, the present project has as main objective the bioprospecting of bacteriocinogenic probiotic lactic acid (BAL) bacteria, present in the intestinal microbiota of swine (Sus scrofa domesticus). For this purpose, biochemical and molecular identification of isolated probiotic bacteria will be performed and the cell-free supernatant will be used in order to evaluate the production, the peptide nature and the antimicrobial power of bacteriocins against Escherichia coli, one of the main swine pathogens. The results obtained will make it possible to identify new safe probiotic strains for use in animal feed, as well as effective bacteriocins to combat pathogenic bacteria.