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Extraction and separation of phenolic compounds from green propolis by a bidimensional system

Grant number: 20/03623-2
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): September 01, 2020
Effective date (End): April 30, 2024
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal researcher:Maurício Ariel Rostagno
Grantee:Letícia Sanches Contieri
Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
Associated research grant:18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2
Associated scholarship(s):21/11023-8 - Development of sustainable methods for propolis extracts by the application of non-volatile alternative solvents, BE.EP.DD

Abstract

Green propolis has high biological potential due to the presence of bioactive compounds. Green propolis has the highest selling price in the international market, suggesting that there is a relationship between its color and its chemical composition, rich in phenolics acids. Advanced extraction techniques should be used to make better use of these compounds. This research project proposes the development of a two-dimensional extraction, separation, and analysis system (PLEgrad-SPE x UHPLC) by coupling extraction with pressurized liquids in gradient (PLEgrad) with Solid-Phase Extraction (SPE) in the first dimension and orthogonally with Ultra-High-Pressure Chromatography (UHPLC) in the second dimension. The objective is to develop a system that allows the characterization of the chemical composition of natural raw materials in a fast, efficient and real-time monitoring while allowing the production of small quantities of extracts obtained through the fractionation of the present compounds. These fractions should be well characterized chemically and with low contamination between them. Initially, rapid UHPLC analysis methods will be developed that will be used in the second separation dimension. Several analysis conditions (temperature, column size, flow, volume, and injection solvent and mobile phase composition) will be evaluated for an ultra-fast separation method (time between samples < 2.0 min). Subsequently, different extraction conditions will be evaluated by SPE coupled gradient PLE, including temperature, pressure, sample amount, adsorbent type, flow, solvent, and duration of the extraction/elution gradiente. (AU)

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