Nanoemulsions containing terpenes as potentials larvicides and repellents against Aedes aegypti (Linnaeus, 1762)

• Grant number: 19/25125-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2020
• Effective date (End): October 31, 2023
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Marlus Chorilli
• Grantee: Jonatas Lobato Duarte
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 21/11487-4 - Repellency and larvicidal efficacy of cymene and myrcene-loaded nano-formulations against resistant and non-resistant Aedes aegypti L., BE.EP.DR

Abstract

Aedes aegypti L. is an insect of great medical importance because it is the transmitter of several diseases that cause significant problems to public health worldwide, such as Dengue, ZiKa, Chikungunya and Malaria. The main ways to prevent infection with these viruses are the use of repellents and larvicidal agents that eliminate insect larvae. Medicinal plant derivatives have been used since immemorial time as insecticides and repellents, especially essential oils (OE). The constituents of EO are terpenes, mainly monoterpenes and sesquiterpenes, low molecular weight substances. Among the insecticidal and repellent terpenes, there are myrcene and cymene. However, they have low water solubility and low stability, characteristics that make their use unfeasible. Thus, nanotechnology emerges as strategy to improve the solubilities of these substances in water, as well as the protection and delivery of these assets. Nanostructured systems include nanoemulsions, dispersions of oil droplets stabilized by an emulsifying agent in water. These systems enable the delivery of assets, improve absorption, allowing suspension of lipophilic assets in aqueous environments. Given the above the aim of this work will be develop nanoemulsions containing cymene and myrcene terpenes with potential larvicidal activity and repellent against Ae aegypti L. A series of formulations will be made using low energy input method, which will be characterized by visual aspect, particle size, polydispersion index, particle number size and density distribution, zeta potential, morphology, reology, encapsulation efficiency, release and skin permeation. The selected formulations will be evaluated for cytotoxicity, toxicity against Galleria and Zebrafish model. The larvicidal and repellent activities against Ae. aegypti mosquito will be verified. It is expected from this work to develop promising formulations with potential for the control of Ae. aegypti L. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DUARTE, JONATAS LOBATO; DI FILIPPO, LEONARDO DELELLO; SOUSA ARAUJO, VICTOR HUGO; DE FARIA MOTA OLIVEIRA, ANNA ELIZA MACIEL; CAVALCANTE DE ARAUJO, JENNIFER THAYANNE; DA ROCHA SILVA, FLAVIA BENINI; PINTO, MARA CRISTINA; CHORILLI, MARLUS. Nanotechnology as a tool for detection and treatment of arbovirus infections. Acta Tropica, v. 216, APR 2021. (18/18488-3, 19/25125-7)

DI FILIPPO, LEONARDO DELELLO; AZAMBUJA, JULIANA HOFSTATTER; PAES DUTRA, JESSYCA APARECIDA; LUIZ, MARCELA TAVARES; DUARTE, JONATAS LOBATO; NICOLETI, LUIZA RIBEIRO; OLALLA SAAD, SARA TERESINHA; CHORILLI, MARLUS. Improving temozolomide biopharmaceutical properties in glioblastoma multiforme (GBM) treatment using GBM-targeting nanocarriers. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 168, p. 76-89, NOV 2021. (20/12622-0, 20/04133-9, 19/25125-7)

NICOLETI, LUISA RIBEIRO; DI FILIPPO, LEONARDO DELELLO; DUARTE, JONATAS LOBATO; LUIZ, MARCELA TAVARES; SABIO, RAFAEL MIGUEL; CHORILLI, MARLUS. Development, characterization and in vitro cytotoxicity of kaempferol-loaded nanostructured lipid carriers in glioblastoma multiforme cells. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 226, p. 7-pg., 2023-04-11. (19/25125-7, 19/17913-5, 20/12622-0)

DI FILIPPO, LEONARDO DELELLO; DE PAULA, MARIANA CARLOMAGNO; DUARTE, JONATAS LOBATO; DE PAULA, GEANNE APARECIDA; FRIGIERI, ISADORA; CHORILLI, MARLUS. Nanocarrier functionalization strategies for targeted drug delivery in skin cancer therapy: current progress and upcoming challenges. Expert Opinion on Drug Delivery, v. 20, n. 6, p. 19-pg., 2023-06-14. (19/25125-7, 19/17913-5, 20/12622-0)

DI FILIPPO, LEONARDO DELELLO; DUARTE, JONATAS LOBATO; AZAMBUJA, JULIANA HOFSTATTER; MANCUSO, RUBIA ISLER; LUIZ, MARCELA TAVARES; SOUSA ARAUJO, VICTOR HUGO; FIGUEIREDO, INGRID DELBONE; BARRETTO-DE-SOUZA, LUCAS; SABIO, RAFAEL MIGUEL; SASSO-CERRI, ESTELA; et al. Glioblastoma multiforme targeted delivery of docetaxel using bevacizumab-modified nanostructured lipid carriers impair in vitro cell growth and in vivo tumor progression. International Journal of Pharmaceutics, v. 618, p. 12-pg., 2022-04-25. (19/25125-7, 18/18488-3)

DI FILIPPO, LEONARDO DELELLO; DUARTE, JONATAS LOBATO; ROQUE-BORDA, CESAR AUGUSTO; PAVAN, FERNANDO ROGERIO; MENEGUIN, ANDREIA BAGLIOTTI; CHORILLI, MARLUS; MELERO, ANA; GUILLOT, ANTONIO JOSE; SPAGNOL, CAROLINE MAGNANI; CORREA, MARCOS ANTONIO. In Vitro Skin Co-Delivery and Antibacterial Properties of Chitosan-Based Microparticles Containing Ascorbic Acid and Nicotinamide. LIFE-BASEL, v. 12, n. 7, p. 14-pg., 2022-07-01. (19/25125-7, 15/19097-0, 16/20957-6, 15/02619-3, 20/16573-3)

DE ARAUJO, JENNIFER THAYANNE CAVALCANTE; TAVARES JUNIOR, ALBERTO GOMES; DI FILIPPO, LEONARDO DELELLO; DUARTE, JONATAS LOBATO; RIBEIRO, TAIS DE CASSIA; CHORILLI, MARLUS. Overview of chitosan-based nanosystems for prostate cancer therapy. EUROPEAN POLYMER JOURNAL, v. 160, NOV 5 2021. (19/19228-8, 19/25125-7, 18/18488-3, 20/06212-3)

SOUSA ARAUJO, VICTOR HUGO; CHAVES DE SOUZA, MAURICIO PALMEIRA; CARVALHO, GABRIELA CORREA; DUARTE, JONATAS LOBATO; CHORILLI, MARLUS. Chitosan-based systems aimed at local application for vaginal infections. Carbohydrate Polymers, v. 261, JUN 1 2021. (19/10261-2, 19/25125-7)

DI FILIPPO, LEONARDO DELELLO; DUARTE, JONATAS; FONSECA-SANTOS, BRUNO; TAVARES JUNIOR, ALBERTO GOMES; ARAUJO, VICTOR; ROQUE BORDA, CESAR AUGUSTO; VICENTE, EDUARDO FESTOZO; CHORILLI, MARLUS. Mucoadhesive Nanosystems for Nose-to-Brain Drug Delivery in the Treatment of Central Nervous System Diseases. Current Medicinal Chemistry, v. 29, n. 17, p. 32-pg., 2022-01-01. (20/12622-0, 19/25125-7, 20/16573-3, 18/23416-1)