Development of biosensors based on microelectrodes for in vitro and in vivo monitoring of neurometabolic markers in the rats brain

• Grant number: 20/11722-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: March 01, 2021
• End date: February 28, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Mauro Bertotti
• Grantee: Daniel Matias Gaston Regiart
• Supervisor: Rui Manuel Silva Gomes Barbosa
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Universidade de Coimbra (UC), Portugal
• Associated to the scholarship: 19/06293-6 - Investigations in biological systems by using miniaturized electrochemical sensors, BP.PD

Abstract

This research project aims to develop integrated systems for real-time monitoring of target compounds or neurometabolic markers of clinical relevance in in vitro and in vivo experiments in the brain of rats. Emphasis will be directed to the following compounds: glucose, lactate, pyruvate, hydrogen peroxide and oxygen. The proposed devices will consist of miniaturized electrochemical sensors, and emphasis will be given to the construction of systems that can be easily used and that have reliable and long-lasting operation. High selectivity and sensitivity in relation to the desired analytes will be achieved with platforms designed based on new materials. The sensors developed will have to satisfy analytical requirements (for example, reduced drift response, resistance to surface poisoning and high selectivity, accuracy and precision). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REGIART, MATIAS; LEDO, ANA; FERNANDES, ELIANA; MESSINA, GERMAN A.; BRETT, CHRISTOPHER M. A.; BERTOTTI, MAURO M.; BARBOSA, RUI. Highly sensitive and selective nanostructured microbiosensors for glucose and lactate simultaneous measurements in blood serum and in vivo in brain tissue. BIOSENSORS & BIOELECTRONICS, v. 199, p. 10-pg., 2022-03-01. (20/11722-0)