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Biochemical and histological evaluation of the effects of direct and remote ischemic preconditioning on the kidney of young rats

Grant number: 20/03925-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2020
Effective date (End): September 30, 2021
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Ana Cristina Aoun Tannuri
Grantee:Arthur Henrique de Almeida Oliveira
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Kidney transplantation is the indicated alternative for patients with end stage chronic renal failure, whether the patient is on dialysis or pre-dialysis. Among the biological obstacles to transplantation, ischemia and reperfusion injury (IR) stands out - IR injury being a condition typified by the temporary restriction of the arterial supply of an organ followed by the reestablishment of perfusion and subsequent reoxygenation. Less exposure to IR injury is known to bring about better immediate renal function in kidneys transplanted or undergoing partial nephrectomy, as well as longer kidney survival in the long term. Thus, the technique of ischemic preconditioning emerged with the prerogative of reducing cellular damage to the organ in ischemic distress. This paper aims to explore the effects of the two previously documented preconditioning techniques, direct (PCID) and remote (PCIR). But beyond that, using young rat will also simulate the differentiated physiology of children. Since the age of these mammals has been little related in the studies already published, this will be an opportunity to acquire updated and age-differentiated data, as well as allowing to evaluate the extent of the benefits and risks of applying either of the two preconditioning techniques, allowing to compare them not just with the control group, but with each other. Being distinguishers of great importance for this project. Forty-nine young rats (60-90 g) are going to be divided into 4 groups: control group, ischemia-reperfusion group, direct ischemic preconditioning group followed by ischemia and reperfusion and remote ischemic preconditioning group followed by ischemia and reperfusion. We will weigh, measure length, serum and histological studies to evaluate the degree of renal impairment, comparing these groups to be able to determine the degree of protective effect of these two techniques. Renal samples will be collected by laparoscopic surgery.