Effect of jabuticaba peel extract on bone tissue of osteoporotic rats

• Grant number: 20/14147-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2021
• End date: January 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Márcio Mateus Beloti
• Grantee: Gabriela Hernandes Campeoti
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Several natural products have been investigated for therapeutic purposes, such as prevention and treatment of damages generated by metabolic disease. Fruits can be important sources of a plethora of bioactive compounds mainly concentrated in their peels. Jabuticaba peel extract (JPE) has been shown to be a promising product in the treatment of obesity, pre-diabetes, and liver complications. Recently, our research group evaluated the effect of JPE on osteoblastic and adipocytic differentiation of mesenchymal stem cells derived from healthy and osteoporotic animals. The results showed that the JPE increases osteoblastic differentiation, evidenced by higher alkaline phosphatase activity and extracellular matrix mineralization, and reduces adipocytic differentiation, observed by lower lipid accumulation, in both systemic conditions. Based on these results, our hypothesis is that due to its osteogenic and anti-adipogenic effects, JPE can reduce bone loss induced by osteoporosis. In this context, the aim of this project is to evaluate the effect of JPE on bone tissue of osteoporotic rats. Rats will be submitted to the surgical procedure to induce osteoporosis (ovariectomy, Ovx) or fictitious surgery (Sham). After 150 days of the surgical procedure, the animals will receive JPE solution dissolved in filtered water (5.8 mg JPE/kg of body weight diluted in 2 mL of filtered water) or 2 mL of filtered water through daily intragastric administration (gavage), up to 60 days. Then, the animals will be euthanized, and the femoral epiphyses will be removed and evaluated by microtomography and histological analyses. The data (n = 12 for each group of animals) will be submitted to the appropriate statistical test. The results of this study may generate therapeutic possibilities for the treatment of diseases that affect bone tissue, since its high availability and low cost would result in clinical applications to the population.